Consider the fluorescence spectra for bovine serum albumin (BSA) below. One solution contains phosphate buffered saline (PBS), while the other contains 1M HCL. Why does the fluorescence change the way it does? I.e., why do we observe a higher peak and a shift to the left under the presence of acid?
Consider the fluorescence spectra for bovine serum albumin (BSA) below. One solution contains phosphate buffered saline (PBS), while the other contains 1M HCL. Why does the fluorescence change the way it does? I.e., why do we observe a higher peak and a shift to the left under the presence of acid?
Human Anatomy & Physiology (11th Edition)
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Author:Elaine N. Marieb, Katja N. Hoehn
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Consider the fluorescence spectra for bovine serum albumin (BSA) below. One solution contains phosphate buffered saline (PBS), while the other contains 1M HCL. Why does the fluorescence change the way it does? I.e., why do we observe a higher peak and a shift to the left under the presence of acid?
 alt="Figure 4: Fluorescence Spectroscopy Emission Spectra of BSA (PBS & Acid)
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BSA-PBS
BSA-Acid
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(327,115.05) (345,111.24)
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Transcribed Image Text:Figure 4: Fluorescence Spectroscopy Emission Spectra of BSA (PBS & Acid)
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BSA-PBS
BSA-Acid
120
(327,115.05) (345,111.24)
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60
40
20
300
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Wavelength (nm)
Intensity (a.u.)
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