Consider the fluorescence spectra for bovine serum albumin (BSA) below. One solution contains phosphate buffered saline (PBS), while the other contains 1M HCL. Why does the fluorescence change the way it does? I.e., why do we observe a higher peak and a shift to the left under the presence of acid?
Consider the fluorescence spectra for bovine serum albumin (BSA) below. One solution contains phosphate buffered saline (PBS), while the other contains 1M HCL. Why does the fluorescence change the way it does? I.e., why do we observe a higher peak and a shift to the left under the presence of acid?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
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Consider the fluorescence spectra for bovine serum albumin (BSA) below. One solution contains phosphate buffered saline (PBS), while the other contains 1M HCL. Why does the fluorescence change the way it does? I.e., why do we observe a higher peak and a shift to the left under the presence of acid?
Transcribed Image Text:Figure 4: Fluorescence Spectroscopy Emission Spectra of BSA (PBS & Acid)
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BSA-PBS
BSA-Acid
120
(327,115.05) (345,111.24)
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20
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Wavelength (nm)
Intensity (a.u.)
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