A chemistry student at GGC synthesized a new sulfonamide (S7) drug to help treat malaria. Using a spectrophotometer the student observed that S7 converts to a colorless molecule in the presence of malaria parasite called Plasmodium falciparum. To study the rate of reaction, the student recorded the absorbance/amount of S7 at a wavelength of 570 nm. The results are summarized in the table below: Absorbance/Amount Time (s) of S7 0.80 0.63 10 0.50 15 0.40
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- Penicillin heralded the dawn of the antibiotic age when it was discovered in 1928 and is now the most widely used antibiotic in the world. A membrane electrode was developed for the quantitative analysis of penicillin. The table above shows the data which were collected using a set of penicillin standards against saturated calomel electrode. An unknown sample produced a potential of 142 mV. A) Which served as the reference electrode? B) What is the molar concentration of penicillin in the sample?You have an order for 1 gram of Cefazolin in D5W 100 ml. You have added 5 ml of sterile water to the 1 gram vial to reconstitute powder. However the recommended manufacturer’s diluent amount is 10 ml of sterile water for a final concentration of 100 mg/ml. How would reconstituting the vial with 5 mls affect the concentration and the final calculated dose. Please answer with explanation ASAP. I will really upvote. ThanksGiven this, if you used 6g of vitamin Z powder to make 20 ml of solution, what is the % concentration of this solution? (I gave the image since I don't know if that info is needed to solve this question.)It also gives a follow-up, if you can help here too: You work in a lab as a summer student. One of your tasks is to make sure that there is enough cell culture medium containing antibiotics to grow bacteria. One day you realize that there is only 5 ml of 10% Antibiotic stock solution in the freezer. You decide to use it all to prepare the working culture medium with 0.01% antibiotic. In the lab there is plenty of growth medium without antibiotics. (Note: dilution in medium is like dilution in water). You remember the equation to make dilutions of stock solutions. You usually use this formula to calculate the required volume of a stock solution, but you realize it can apply here as well, even though the unknown is the final volume. So, you make that dilution. Given that each bacterial…
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- In the context of flow cytometry data: The Lactobacillus cells also show a clear increase in PerCP (red) fluorescence when stained with SYBRGreen (SG) compared to unstained cells. Why might this be the case? Think about the emission spectrum of chlorophyll and SG in combination with the dichroic mirrors (e.g. the ‘filters’ for the detectors).Nanoparticles were added in a liquid to prompt the medication release, and it was observed that 5 mg of the medication was released in 1 liter of PBS, which accounted for 60% of the total amount of the drug. While producing an equivalent number of nanoparticles, 5 ml of 10 mg ml−1 of the drug was added. What is the efficiency of encapsulation?In UV/Visible spectrophotometer analysis for a multicomponent system, there are only two dyes used in the mixture, the two proportions should be totalled to 1.0. but on finding You got 0.6 in total. Explain the reasons for the difference.
- The figure above depicts an agar cube with a side length of 13\, \text{mm}13mm13, start text, m, m, end text. In an experiment, students submerged the cube in red dye for 121212 hours. The red dye permeated 1\, \text{mm}1mm1, start text, m, m, end text on each side, as indicated by the shading in the figure. Volume of a rectangular solid: V = lwhV=lwhV, equals, l, w, h Calculate the volume of the agar cube that remained unpenetrated by the red dye.You may want to use this resource for this problem. If you do, submit the output along with your solution.You have been given a confocal microscope equipped with the following lasers, excitation filters, andemission filters:Laser Emission filter355 nm 410-470 nm405 nm 470-500 nm488 nm 500-550 nm532 nm 570-610 nm561 nm 610-650 nm640 nm 660-700 nm808 nm 720-780 nmYour task is to design an experiment to visualize the following:1. Nuclei2. A fluorescent protein in the cytosol3. A cell membrane marker antibody conjugated with a fluorophore4. Actin filaments5. LysosomesYou may choose from the following fluorophores for each of the five channels:Nuclei Fluorescent protein Membrane marker Actin marker Lysosome trackerDAPI GFP FITC AF488 Phalloidin LysoTracker RedHoechst 33342 YFP WGA-TRITC AF568 Phalloidin LysoTracker DeepRedSYTO Deep Red RFP Cy7 AF594 Phalloidin LysoTracker Blue Part 3.1Choose appropriate fluorophores for each of the subcellular structures to be imaged, taking into…in food microbiology, how do you compute for concentration (M) and absorbance (A) of peroxidase activity on hydrogen peroxide at different pH values? (wavelength used = 415nm). can you please explain the calculations thank you if given: Temperature = 75 C Molar absorptivity coefficient = 10.5 Path length (cm) = 2 I = transmitted light = 0.45