The authors of this paper also tested another minimal media (Media C) which is exactly the same as Media B except that the pH is 7.4 instead of 7.1. Which media components would be different in Media C? For each component that is different, state if will be larger or smaller than the amount in Media B.

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The authors of this paper also tested another minimal media (Media C) which is exactly the same as Media B except that the pH is 7.4 instead of 7.1. Which media components would be different in Media C? For each component that is different, state if will be larger or smaller than the amount in Media B.

It is sometimes useful to grow bacteria in what is called a "minimal media". Minimal media is a
carefully constructed solution that contains the absolute minimum number of substances needed for
bacteria to grow. In 2019, Stephan B Azatian, Navneet Kaur, and Michael P Latham reported a new
minimal media. Here is the composition of the new media and the media that they had been using
previously.
Table 1: Composition of minimal media.
The listed quantities are for 1 L of media.
Media A
Media B
Chemical
(Original)
(New)
Na2HPO4
6.8 g
13.6 g
KH2PO4
3 g
|6g
NaCl
0.5 g
1g
MgSO4 • 7H2O
0.25 g
0.5 g
(NH4)2SO4
1g
1g
d-Glucose
3 g
3 g
Trace metals
solution
0.5 mL
0.5 mL
Vitamins
1 mL
1 mL
a. Media recipes from Azatian SB, Kaur N, Latham MP. Increasing the buffering capacity of minimal
media leads to higher protein yield. J Biomol NMR. 2019;73(1-2):11-17. doi:10.1007/s10858-018-
00222-4
Transcribed Image Text:It is sometimes useful to grow bacteria in what is called a "minimal media". Minimal media is a carefully constructed solution that contains the absolute minimum number of substances needed for bacteria to grow. In 2019, Stephan B Azatian, Navneet Kaur, and Michael P Latham reported a new minimal media. Here is the composition of the new media and the media that they had been using previously. Table 1: Composition of minimal media. The listed quantities are for 1 L of media. Media A Media B Chemical (Original) (New) Na2HPO4 6.8 g 13.6 g KH2PO4 3 g |6g NaCl 0.5 g 1g MgSO4 • 7H2O 0.25 g 0.5 g (NH4)2SO4 1g 1g d-Glucose 3 g 3 g Trace metals solution 0.5 mL 0.5 mL Vitamins 1 mL 1 mL a. Media recipes from Azatian SB, Kaur N, Latham MP. Increasing the buffering capacity of minimal media leads to higher protein yield. J Biomol NMR. 2019;73(1-2):11-17. doi:10.1007/s10858-018- 00222-4
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