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As bacteria grow, they produce acidic metabolic waste products that gradually slow down the rate of cell division. Based on this, which buffer would you expect to allow the bacteria to grow for a longer period of time

As bacteria grow, they produce acidic metabolic waste products that gradually slow down the rate of cell division. Based on this, which buffer would you expect to allow the bacteria to grow for a longer period of time

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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As bacteria grow, they produce acidic metabolic waste products that gradually slow down the rate of cell division. Based on this, which buffer would you expect to allow the bacteria to grow for a longer period of time before slowing down? Include the reasons supporting your prediction.

**Table 1: Composition of Minimal Media** The listed quantities are for 1 liter (L) of media. | Chemical | Media A (Original) | Media B (New) | |---------------------|--------------------|---------------| | Na₂HPO₄ | 6.8 g | 13.6 g | | KH₂PO₄ | 3 g | 6 g | | NaCl | 0.5 g | 1 g | | MgSO₄ · 7H₂O | 0.25 g | 0.5 g | | (NH₄)₂SO₄ | 1 g | 1 g | | d-Glucose | 3 g | 3 g | | Trace metals solution | 0.5 mL | 0.5 mL | | Vitamins | 1 mL | 1 mL | **Description:** This table outlines the composition differences between two types of minimal media, labeled as Media A (Original) and Media B (New). Each component's quantity is presented in grams (g) for solids and milliliters (mL) for liquids, per liter of media. Media B contains increased amounts of Na₂HPO₄, KH₂PO₄, NaCl, and MgSO₄ · 7H₂O compared to Media A, while the quantities of (NH₄)₂SO₄, d-Glucose, trace metals solution, and vitamins remain unchanged.
Transcribed Image Text:**Table 1: Composition of Minimal Media** The listed quantities are for 1 liter (L) of media. | Chemical | Media A (Original) | Media B (New) | |---------------------|--------------------|---------------| | Na₂HPO₄ | 6.8 g | 13.6 g | | KH₂PO₄ | 3 g | 6 g | | NaCl | 0.5 g | 1 g | | MgSO₄ · 7H₂O | 0.25 g | 0.5 g | | (NH₄)₂SO₄ | 1 g | 1 g | | d-Glucose | 3 g | 3 g | | Trace metals solution | 0.5 mL | 0.5 mL | | Vitamins | 1 mL | 1 mL | **Description:** This table outlines the composition differences between two types of minimal media, labeled as Media A (Original) and Media B (New). Each component's quantity is presented in grams (g) for solids and milliliters (mL) for liquids, per liter of media. Media B contains increased amounts of Na₂HPO₄, KH₂PO₄, NaCl, and MgSO₄ · 7H₂O compared to Media A, while the quantities of (NH₄)₂SO₄, d-Glucose, trace metals solution, and vitamins remain unchanged.
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