How much liquid media should you put in your dilution blank if you wanted to serially dilute a1 ml sample by a factor of 5?

Q: What is the purpose of sealing the flask with a balloon? What product is captured in the balloon?

A: Fermentation is the process of ATP generation by the microorganism. Carbohydrates are the substrate…

Q: List the steps that you implement while using oil immersion to observe the specimen.

A: An instrument that helps to see very small objects is known as a microscope. There are different…

Q: My question is: Could you explain steps? (etc. why do we heat or add chemicals like nacl or ammonium…

A: Casein is the major protein component found in milk. It is generally a phosphoprotein which is…

Q: Does measuring using higher (50mL vs 100mL) produce more accurate and precise results?  Why?

A: In the realm of scientific measurements and laboratory practices, the choice of container size can…

Q: Given: The used ocular objective while taking this image, has magnifying power of 6x The used…

A: Given : Stage micrometer used has graduation = 0.01mm Ten graduation on ocular micrometer coincides…

Q: What is the final dilution factor if you do five a) 0.625 b) 3.1x10^6 c) 6x10^-6 d) 2.67x10^7 e)…

A: Serial dilution is a technique used to create a series of dilutions from a concentrated stock…

Q: To prevent inclusion from occurring during precipitation, the technique to follow is I. filter…

A: When an impurity takes up a lattice site in the carrier's crystal structure, inclusion takes place.…

Q: Why Purified synthetic alcohol is preferred for level vial?

A: An alcohol is an organic compound that carries at least one hydroxyl functional group (-OH) bound to…

Q: Why is there a need to filter the alkaline solution after adding the Barium chloride solution?

A: In an aqueous solution, barium chloride acts as a simple salt.  However when dissolved in water, it…

Q: Answer the following questions based on Kirby Bauer / MacFarland standards 1-0.5 McFarland…

A: The Kirby Bauer test, also known as the disc diffusion test, is a common laboratory technique for…

Q: Why would the optical tweezers be a method superior to dilution and liquid enrichment for obtaining…

A: Optical tweezers have been used to trap dielectric spheres, viruses, bacteria, living cells,…

Q: Why is it necessary to flame the top of the bottle before pouring the plate?

A: Aseptic techniques are used to prevent contamination from pathogens. These are used in clinics and…

Q: What is the purpose of the oil when using oil immersion objective? 2. Why should the oil…

A: A microscope is a device that magnifies small objects. Some microscopes can even be used to view an…

Q: a)Determine the amount of X (in gram) in order to prepare 100 mL of 50 mM stock solution of X. Show…

A: In this question, we are given: a) Molecular weight of X= 225 Molarity of stock solution= 50 mM…

Q: What is the meaning of the different values given engraved on each objective?

A: Microscopes are used in viewing microscopic organisms, including bacteria, parasites, etc., and also…

Q: What is the underlying basis for the use of oil when using an oil immersion objective?

A: Microscopy is the technical field of using microscopes to view samples and objects that the naked…

Q: What is the color result of 1% solution of casein, Albumin, Gelatin in ninhydrin test? Include…

A: Ninhydrin test: Chemical test to detect presence of ammonia, primary/secondary amines, or amino…

Q: What do to do when the disstillation did not make the sample completely pure? What could have went…

A: Distillation is a process that is based upon the boiling points of various liquids present in the…

Q: PREPARATION OF SMEARS: 1. Wash the slide thoroughly with a detergent, rinse with water and dry with…

A: PREPARATION OF SMEAR ( FLOWCHART)

Q: What is the concentration of a 50% solution after completing three 1:10 serial dilutions?

A: Serial dilution is the process that reduces the concentration of a solution in an organised manner.…

Q: What are 2 advantages to using absorbance values (from a Spectrophotometer) over a spread plate and…

A: Introduction: Spectrophotometry is a method utilized for the evaluation and portrayal of drab mixes…

Q: Why is it necessary to use immersion oil when the 100X objective is used'

A: Note - we answer one question at a time. Immersion oil is transparent and has specific viscosity and…

Q: What is the likely result if you try to use the 100X objective without immersion oil?

A: Introduction :- To prevent any air gaps and light loss owing to refraction (bending of the light)…

Q: Why is there a need to use acids and boil the sample in maceration?

A: Maceration is a common method of obtaining medicinal plants, it is an extraction process in which…

Q: What is one advantage of a pour plate over a streak plate?

A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…

Q: what is oil immersion and why is it used

A: Light microscope is a microscopic instrument which uses visible light and magnifying lens to to view…

Q: f you diluted 200 uL of your culture into 1800 uL of water what is your dilution factor? (Show your…

A: A dilution factor is a ratio that represents the change in concentration of a solution after it has…

Q: Given: The used ocular objective while taking this image, has magnifying power of 6x The used…

A: Magnification on a microscope denotes the amount or degree of visual enlargement of an observed…

Q: 1. One gram of sludge was added to a 99 mL dilution blank. Three 1/10 dilutions were then made. From…

A: Colony-forming units per milliliter, or CFU/ml, are measured in the sample. It provides us with an…

Q: What is the importance of the Nitrate test? What information can we gather from this test? Please…

A: Nitrate broth used in the nitrate test contains nutrients and potassium nitrate as a source of…

Q: Voges Proskauer test Does color of the medium change to red after both reagents were added and…

A: Biochemical method of bacterial identification is a classical method that is used for the…

Q: the image contains results of the fluid thioglycolate medium tests. Please explain the results and…

A: Fluid thioglycolate medium test It is a multipurpose and enriched test. It is a differential medium…

Q: When loading samples onto a gel, why should expelling all the liquid in the tip be avoided?   a)All…

A: It is a laboratory tool commonly used in biotechnology and medicine to transport a measured volume…

Q: What special precautions must be taken when using the oil immersion (100x) objective?

A: Using oil immersion reduces the air gap between the glass slide and the objective lens. This air gap…

Q: How can you make a final dilution of 10-10  using 5 dilution tubes? Outline your steps.

A: Dilution is generally done to decrease the concentration of a solute in a solvent.

Q: How many bacteria would be in 1ml of the sample if you counted 134 colonies on the 1:1,000 dilution?

A:

Q: How many grams of media would be required to prepare 350 plates assuming 20 mL per plate? O 72 O 112…

A: The preparation of  mentioned Escherichia coli culture media contain 36 grams per litre deionized…

Q: A nurse reconstituted a vial of 750 mg Cefuroxime Sodium Powder for Injection with 6mL of sterile…

A: Cefuroxime is an antibiotic and is a type of second generation cephalosporin and used for treatment…

Q: Food dye Initial concentration (µM) | Initial volume (mL) Final concentration (µM) | Final volume…

A: Given Values: Red 40: Initial Concentration (Ci) = 32 μM Initial Volume (Vi) = 1 ml Final…

Q: Give a vivid outline of the Standard operating procedure of neutralization test.

A: The neutralization test is a diagnostic test helps to measure the patient's ability to protect cell…