Answered: How much liquid media should you put in…

How much liquid media should you put in your dilution blank if you wanted to serially dilute a1 ml sample by a factor of 5?

Principles Of Radiographic Imaging: An Art And A Science

6th Edition

ISBN:9781337711067

Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Chapter19: Film And Screens Imaging And Processing

Section: Chapter Questions

Problem 8RQ

See similar textbooks

Related questions

Q: What are the processes of concentrate samples analysis in Uv-visible spectroscopy?

A: UV-visible spectroscopy is a fast and simple technique that finds the analyte concentration of a…

Q: to dilute a sample by 1/3. For a final volume of 27 ml, how much sample would you add to how much…

A: The concentration of the solution fluctuates as it is diluted, but the number of moles of reactant…

Q: You were instructed to add 0.2 ml of kombucha to 9.8 ml of fresh tea, but instead added 2.0 ml of…

A: Dilution is defined as the ratio of stock volume to the final volume. Here, final volume is the sum…

Q: How would you prepare a 10-7dilution from a culture? Each of your dilution tubes cannot hold more…

A: Dilution is the process of lowering a solute's concentration in a solution, typically by mixing it…

Q: Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL…

A: Serial dilution is a progression of successive dilutions used to lessen a thick culture of cells to…

Q: Does measuring using higher (50mL vs 100mL) produce more accurate and precise results? Why?

A: In the realm of scientific measurements and laboratory practices, the choice of container size can…

Q: Why is dilution a necessary part of pure culture preparation?

A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…

Q: Before inoculating and pouring molten nutrient agar into a plate, why must the agar first be cooled…

A: Agar or agar-agar is a jelly-like substances obtained from red algae. Agar is a solidifying agent…

Q: Why should the use of nichrome or iron containing loop/wire be avoided as an inoculating device when…

A: The oxidase is a test used in microbiology to identify bacteria that produce cytochrome c oxidase.…

Q: What do to do when the disstillation did not make the sample completely pure? What could have went…

A: Distillation is a process that is based upon the boiling points of various liquids present in the…

Q: You count 4 squares of a hemacytrometer and ontain 82,65,85 cells in each squy.what is the cell…

A: Hemocytometer is device used for the counting of blood cells in a sample. It has counting chambers…

Q: Using 25 μL of serum, how much diluent would be needed to create a 1:2 dilution? ______ μL

A: The process of reducing a solute concentration by adding solvent to a solution is referred to as…

Q: Why is it necessary to use immersion oil when the 100X objective is used'

A: Note - we answer one question at a time. Immersion oil is transparent and has specific viscosity and…

Q: What is meant by the term plating efficiency?

A: The cells are cultured over the plate at the particular growth conditions within a particular time…

Q: If I am making 500ml of a solution with 0.625ml ITS, 0.0035ml Linoleic acid, 20ml Nu-serum, 0.75g…

A: A homogeneous mixture of two or more substances is referred to as a solution in chemistry when the…

Q: To measure the culture temperature, why is the thermometer placed in a tube separate from the…

A: A microbe or microorganism is a microscopic organism that is too small to be seen with the naked…

Q: You are asked to add 2 mL of bacterial culture to 98 mL of water. What is your dilution factor? Show…

A: Introduction :- A test to determine whether you have a bacterial infection is a bacterium culture.…

Q: If you transferred 4 mL of an undiluted sample into 96 mL of diluting fluid, what dilution have you…

A: 4ml undiluted sample 96ml diluted sample To calculate the dilution ,the formula is C1V1=C2V2. Here…

Q: A sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it.…

A: A serial dilution is a process of sequential dilution in order to decrease the density of cell…

Q: a sample was diluted by placing a 0.1 mL of dilutent and 1 mL of this dilution was pour-plated.…

A: The CFU/ml in the Original Culture

Question

How much liquid media should you put in your dilution blank if you wanted to serially dilute a1 ml sample by a factor of 5?

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 2 steps with 1 images

SEE SOLUTION Check out a sample Q&A here

Similar questions

How would you perform a 100-fold dilution, if you want the final volume to be 15mL? What volume of sample and volume of dilute would be used?
You want to dilute a sample by 1/3. For a final volume of 27 ml, how much sample would you add to how much liquid media?
An original sample of water containing 4.00 X 106 CFU/mL was diluted by 4 successive 1/10 dilutions. After incubation, 200 colonies were found growing on the plate. How many mLs from the last dilution tube were plated out?
a) What is the dilution factor if you add a 1ml aliquot of a specimen to 99ml of diluent? b)How to make 500ml of a 1:250 dilution?
An original sample of water containing 4.20 X 109 CFU/mL was diluted by 4 successive 1/100 dilutions. Exactly 1 mL from the last dilution was used to prepare a pour plate. After incubation, how many colonies were present on this plate?
How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material? Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.
The following image is a scheme for serial dilutions prepared for spectrophotometric analysis. If the stock solution concentration is 0.05 % (v/v) can you calculate the other tube’s concentrations in % v/v? I've used this with direct dilutions, how would I use this on serial dilutions?
a pure bacterial culture was diluted by adding a 0.2 mL aliquot to 0.9mL water. Then 0.1 mL of this dilution was plated out, yielding 82 colonies. Calculate the CFU/mL in the original culture.
If you transferred 4 mL of an undiluted sample into 96 mL of diluting fluid, what dilution have you created? 2/100 O 1/24 1/25 1/20
A sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it. Assuming you plated the same volume on each plate, how many colonies would you expect to find on the 10^-4 plate? Include units and use OCD formula.
What would your final dilution be if you transferred 0.1 mL of a 10-6 dilution and added it to a sterile petri dish and then added molten agar to make a pour plate?
You aseptically transfer 1 ml of your optional liquid culture into 99 ml of sterile water. What is the dilution factor. Show work