You have been given a stock solution of dye that has a 10X concentration. You wish to make750ul of a 1X dilution of this dye. Give the volume of 10X dye that you will use (in ul) You have been given a stock solution of dye that has a 10X concentration. You wish to make870ul of a 1X dilution of this dye. Give the volume of dye that you will use (in ul)

Dilution is the process by which we can reduce the given concentration of the solution by adding…

Answered: You have been given a stock solution of…

You have been given a stock solution of dye that has a 10X concentration. You wish to make 750ul of a 1X dilution of this dye. Give the volume of 10X dye that you will use (in ul)

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

See similar textbooks

Related questions

Q: We’re back in the lab having fun! Our current experiment calls for us to treat our cells with THC…

A: A stock solution is prepared by weighing out an appropriate portion of a solid or by measuring out…

Q: You are asked to prepare 20 mL of a 50 mg/mL drug dilution from a stog of 2 g/5 mL. How many…

A: Here, We need to calculate the milliliter of dilutant needed, for that purpose stock solution…

Q: As shown in the image below, 10 µl of a stock solution (tube #1) are serially diluted into 0.990 ml…

A: Introduction Dilution is the process of lowering the concentration of a solute in a solution by…

Q: For in the solution part for c), do you mean 6.25%?

A: Alleles are the alternative forms of a gene that are located on the same locus of a homologous…

Q: A 500 mg vial of Kefzol needs to be reconstituted to a final concentration of 275 mg/mL. Assuming…

A: Answer. Given data :----- Kefzol vial concentration = 500 mg. Desire concentration of kefzol =…

Q: You own a compounding able pharmacy and are presented with a presciption for fortified gentamicin…

A: The concentration of Gentamicin injection is 40 mg/ml and gentamicin eye drop concentration is 3…

Q: We want to test different concentrations of an antibiotic to see how well it can kill bacteria on a…

A: Introduction:- The Agar dilution of the method is the currently the reference that standard the…

Q: How is 10^4 derived? Please show all calculation steps. # of cells per mL = (Average)*DF*10^4 %3D

A: HEMOCYTOMETER: It is the tool used in most of the biochemistry and biology labs in order to count…

Q: In a neutral solution, the [H] is : O 1.0x10-14M zero 1.0×107M 1.0x10-7M

A: The acidity of any solution is determined by the molarity of the Hydrogen ions. So, the pH of any…

Q: A pharmacist needs an IV which is 8% (w/v) dextrose in water. How many cc of pure sterile water…

A: Introduction:- We have been given initial volume as 500cc which means 500ml of D10W ( 10%) and we…

Q: You record the absorbance of your unknown BSA sample at 0.276. What is the concentration in ug/mL of…

A: Standard curve can be used to determine the concentration of unknown sample by determining their…

Q: Provide a chemical test/reagent that would differentiate each of the following pairs of compounds.…

A: Asked : Reagent and indication of positive test

Q: A student who was isolating aspirin stopped the experiment after the filtration step with alumina.…

A: The melting point is considered as the temperature at which materials changes its form from another…

Q: The order is for Moxam 5 gm IM every 8 hours. On hand is 10 gram of Moxam with the following…

A: Medication order is the terms used to define the directions for a drug needed to be given to the…

Q: Given an 8 M potassium chloride stock solution, explain whether it is possible to perform a dilution…

A: The dissolution of one mole of a compound or solute in a solution with a total volume of one litre…

Q: You are running a size exclusion column to purify your 25 kDa protein from a lysate mixture. The…

A: Size exclusion column chromatography: As a function of their respective sizes, molecules are…

Q: Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.204 and A650=0.061, taking into…

A: Phycocyanin is a blue pigment found in cyanobacteria and certain algae. It is a phycobiliprotein…

Q: How many mL of the 1 M glucose stock solution do you need to prepare 100 mL of a 1 mM glucose…

A: After dilution, the dilution factor represents the number of times the original concentrated stock…

Q: You will be given 1 mL of a 2.0 mg/mL solution of bovine serum albumin (BSA) and 1 mL of a 2.0 mg/mL…

A: Dilution in biochemistry is used to lower and get the desired concentration of solute solution by…

Q: The prescriber ordered oxacillin 500mg IM q6h. The instructions on the 2g vial state to…

A: The nurses administer the drugs to the patient, and thus it is very much necessary for the nurses to…

Q: Prepare a standard tube that contains 0.2 mL of 2.5 mM lysine. To this tube, add 0.1 mL of the…

A: Final concentration can be calculated from the formula C1V1=C2V2 Where C1= initial concentration V1=…

Q: A pharmacist prepares 80 mL of an isotonic solution of 1.1 %w/v Lidocaine HCl (E-value 0.2). To…

A: The ability of a solvent to cause cells to shrink or swell is referred to as tonicity. A…

Q: When conducting a flame test for sodium iodide, is the result positive or negative?

A: An ionic compound is one that is formed between a metal or metalloid and a non-metal. A Flame test…

Q: You receive an order for 750 mL of 27% dextrose solution. You have available D50W, 1000 mL. How many…

A: Given, D50W means 50% dextrose solution, 100 ml 750 ml of 27% dextrose is desired Sterile water

Q: Infuse heparin at 1,200 units per hour from a solution containing 40,000 units of heparin in 500 mL…

A: Introduction:- Heparin is an anticoagulant medication. It is a naturally occuring glycosaminoglycan…

Q: A vial containing 400 mg hydrocortisone sodium succinate powder for injection is to be reconstituted…

A: GIVEN: Amount of hydrocortisone sodium succinate powder for injection = 400 mg Volume of injection =…

Q: Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.211 and A650=0.086, taking into…

A: Phycocyanin is a protein-pigment complex that belongs to the family of phycobiliprotein. It is a…

Q: The aliquot method was used to obtain 8 mg of a drug with a pr

A: Introduction:- the amount of the drug that is weighed that is within the degree of accuracy that…

Q: If equal portions of tretinoin gel, 0.1 % w/w and 0.4 % w/w are combined, what would be the…

A: The objective of the question is to find the resultant percentage strength when equal portions of…

Q: You want to feed a new flask @: 2 x 105 cells/mL, 8 mL total per flask. Describe how you dilute your…

A: Viable or the live cells can be counted by the instrument called the hemocytometer. These live cells…

Question

You have been given a stock solution of dye that has a 10X concentration. You wish to make
750ul of a 1X dilution of this dye. Give the volume of 10X dye that you will use (in ul)

You have been given a stock solution of dye that has a 10X concentration. You wish to make
870ul of a 1X dilution of this dye. Give the volume of dye that you will use (in ul)

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1: Introduction

VIEW

Step 2: About dilution factor

VIEW

Solution

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 3 steps

SEE SOLUTION Check out a sample Q&A here

Similar questions

What is the concentration of a 50% solution after completing three 1:10 serial dilutions?
Each liter of an intranasal antibiotic solution contains 160 mg of gentamicin. You have 2-mL gentamicin vials that contain 40 mg. How many vials will you need if you are to prepare three liters of the antibiotic solution?
To make 100 ml 0.3% NP-40 solution for FISH post washing, how many ul 100% NP-40 solution is needed?
The data from thin layer chromatographic separation of analgesics showed ibuprofen distance to be 0.752 mm. If the solvent distance is 1.85 mm. Calculate the retention factor for Ibuprofen?
Calculate the IV rate in drops per minute for a 1 L bag of Lactated Ringers solution running at 50ml/he using the 15 drop factor?
Consider a mixture of two proteins with molecular weights of 20,000 and 200,000. For simplicity of calculation, both may be approximated as unhydrated spheres with VB 0.740 mL g1. a. Calculate the ratio of the sedimentation coefficients of the proteins in the same medium (density=1.05 g mL¯1) at the same temperature.
You have a stock solution of 40% hydrogen peroxide. You need to make several dilutions for a calibration curve. Calculate how much volume of the stock hydrogen peroxide you need to make 50 mL of 11% hydrogen peroxide.
If you have an absorbance of 2.0 mL of stock dye, what kind of a dilution is needed to get it to 1.0? How do you go about this dilution and how do you calculate?
How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material? Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.
Calculate the appropriate volume (in µL) of 3X loading buffer that should be added to 24.0 µL of a sample prior to loading the sample on an agarose gel.
From a 50% solution of dextrose, make 500 ml of a 5% solution
A 510 mL IV bag contains a 25% dextrose solution. How much of the original solution must be replaced with a 65% dextrose solution to increase the original concentration to 35%?