A student who was isolating aspirin stopped the experiment after the filtration step with alumina. One week later, the methanol was evaporated and the experiment was completed. The melting point of the aspirin was found to be 110–115°C. Explain why the melting point was low and why the melting range was so wide.

Q: We’re back in the lab having fun! Our current experiment calls for us to treat our cells with THC…

A: A stock solution is prepared by weighing out an appropriate portion of a solid or by measuring out…

Q: Using a pipette, add 2 mL of suspended DEAE SEPHAROSE (anion) resin to a chromatographic column.…

A: DEAE Sepharose matrix is used in Anion exchange column. In the given method power of salt strength…

Q: We want to test different concentrations of an antibiotic to see how well it can kill bacteria on a…

A: Introduction:- The Agar dilution of the method is the currently the reference that standard the…

Q: What is the purpose of doing the silver nitrate test? Write the chemical equation involved here

A: Introduction:  Silver nitrate is an inorganic compound and its formula is AgNO3 with a molar mass…

Q: Explain how gel filtration chromatography works. What type of gel will you used when the protein…

A: Chromatography is a process for separating components of a mixture based on their differential…

Q: A pharmacist needs an IV which is 8% (w/v) dextrose in water. How many cc of pure sterile water…

A: Introduction:-  We have been given initial volume as 500cc which means 500ml of D10W ( 10%)  and we…

Q: (a) You decide to purify the 5-HT 2A receptor. The protein has a predicted molecular Mass of 46.54…

A: Formation of the peptide bond neutralises any charge due carboxyl group, therefore the net charge on…

Q: how many ml of sterile water injection must be mixed with two 4 ml vials of sterile hypertonic NaCl…

A: Tonicity refers to the ability of a solvent to cause cells to shrink or swell. A semipermeable…

Q: Three dipeptides are separated by electrophoresis at ph 5.8. if the dipeptides are Arg-trp, Asp-thr,…

A: Electrophoresis describes the separation of charged particles and their migration in the influence…

Q: if you had a protein sample solution of unknown concentration which gave an absorbance of 0.992…

A: Answer:- The standard curve gives the absorption value of aliquots having the substance of known…

Q: What is the concentration of cells in the original cell suspension?

A: we can use the dilution formula:C1 V1 = C2 V2where:EXPLANATIONC1 is the initial concentration of the…

Q: The order is for Moxam 5 gm IM every 8 hours.  On hand is 10 gram of Moxam with the following…

A: Medication order is the terms used to define the directions for a drug needed to be given to the…

Q: If you have to prepare 4 nM solution of human IgG ( immunoglobuline) which has molecular mass of 150…

A: The number of moles of a solute present in one liter of the solution is called the molarity of that…

Q: Why does water separate out from a gel after freezing?

A: On freezing of gel some damage occur to gel. damage occur to polymer and colloids that binds the gel…

Q: Why does adding acetic acid to the milk improve the extraction efficiency of the pentobarbital? You…

A: In liquid–liquid (solvent) extraction, the solute partitions itself between two immiscible phases.…

Q: Since the development of the calcium chloride transformation procedure, other procedures have been…

A: Bacterial transformation is the transfer of  DNA released from a donor bacterium into the…

Q: Given 10 gram of vitamin C (E=0.18), we can make ml of isotonic Vitamin C solution.

A: Isotonic solution has the same water concentration and solute concentration as in the cytoplasm of…

Q: You have a stock solution of a tubulin antibody. You were instructed by your teaching assistant to…

A: In laboratory research and experiments, accurate dilutions of solutions are often required to…

Q: After doing the preliminary studies on redcrest protein extract, Tighnari proceeded with its…

A: Proteins are macromolecule comprised of amino acids linked by peptide bonds which forms a primary…

Q: A scientist tries to harvest bacterial cells by centrifugation; he used an RPM of 5000. If the…

A: Centrifugation is a process in which molecules are segregated out at high speed but as different…

Q: Using ultrafiltration, you concentrate a solution of Protein X as follows: Initial volume protein X…

A: Ultrafiltration is a laboratory technique used to remove minute-level impurities from biological…

Q: You want to feed a new flask @: 2 x 105 cells/mL, 8 mL total per flask. Describe how you dilute your…

A: Viable or the live cells can be counted by the instrument called the hemocytometer. These live cells…

Q: How should you prepare your running buffer?

A: SDS-PAGE is method of separation of protein based on its size. Running buffer of the SDS-PAGE…

Q: The commercial hydrogenation of vegetable oil often leads to a trans acid. Explain this statement.

A: Fat hydrogenation is the process of combining fat typically vegetable oils with hydrogen in order to…

Q: Gel-filtration chromatography separates molecules according to their size . Smaller molecules…

A: Gel-filtration chromatography is a type of separation technique that uses a gel as a medium through…

Q: Figure 3: ● • . ● KDa ● 97.4 66.2 45.0 ● 31.0 21.5 14.4 S-1 p-1 2.8 2-d S.3 p-3 This figure was…

A: The study of protein behavior under different conditions is a fascinating aspect of molecular…

Q: You're trying to purify a protein with the aid of an ion exchange column. On the column, the protein…

A: The most commonly used technique to separate and purify proteins, nucleic acids, polypeptides and…

Q: a) mentioned the name of simple laboratory method to roughly estimate the concentration of an…

A: Proteins have wide variety of essential function in our body like providing energy, structural…

Q: What does translucent spot mean with regards to lipid? Identify the control for this test.  1. Oil…

A: Introduction  Oils and fats are a reliable source of energy. A certain amount of human body weight…

Q: A student performed an invertase activity assay on samples from a purification.  All reactions…

A: Beer Lamberts law relates absorbance with concentration of analyte. The Beer lamberts expression is…

Q: When pure hemoglobin (molecular mass of 64,000 Da) is separated by the following methods, what major…

A: Hemoglobin is a tetramer composed of two alpha and two beta subunits. The Molecular weights of the…

Q: Gel Running Buffer is made and kept at 14X concentration for storage. We will need 1.5 L of this…

A: Electrophoresis is a technique in which desired molecule is seperated when electric field is applied…

Q: In solubility test, why do we need to put the lipid samples in a direct heat after adding ethanol?

A: In solubility test, why do we need to put the lipid samples in a direct heat after adding ethanol?…

Q: separating gel

A: here they talk about ion exchange chromatography and electrophoresis. Ion exchange chromatography…

Q: Five dialysis bags, impermeable to sucrose, were filled with various concentrations of sucrose and…

A: Five dialysis bags with varying sugar concentrations are provided. However, the bag's membrane is…

Q: Determine the concentration of monocytogenes enumerated from the apple slice based on the…

A: Microbiology is the study of microorganisms such as bacteria, archaea, viruses, fungi, prions,…

Q: Using 10 μL of a patient's serum, how much diluent would be needed to create a 1:10 dilution? ______…

A: Dilution is the technique of lowering the concentration of a solute in a solution by merely adding…

Q: You have a 50X stock solution of TAE buffer, but you need 2 L of a 1X solution for electrophoresis.…

A: TAE buffer used in agarose gel electrophoresis for the seperation of DNA and RNA.

Q: For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much…

A: RBC is the red blood cell and RBC suspension is normally used in serological testing and are usually…

Q: Explain why protein purification procedures: normally carried out 4-5°C. are

A: Crude extracts  that contain the protein of interest should be refrigerated, and the recombinant…