separating gel
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Which of the following options shows the correct order of fastest to slowest motion of chloride ions, glycine and proteins through the separating gel?
Chloride ions > Proteins > Glycine
Glycine > Chloride ions > Proteins
Chloride ions > Glycine > Protein
Protein > Chloride ions > Glycine
You are trying to separate a mixture of AMP, ADP, and ATP using ion exchange chromatography. Select appropriate supplies for your experiment.
A positively charged column, deprotonated buffer, NaCl of same concentration.
A positively charged column, protonated buffer, NaCl of increasing concentration.
A negatively charged column, protonated buffer, NaCl of increasing concentration
You cannot separate
nucleotides using ion exchange chromatography.
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