You have 12 mL of cells you need to treat with Hydrogen Peroxide (H2O2), such that the final concentration of H2O2 is 50 uM. How much of a 30 mM stock solution of H2O2 would you add to your cells?
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- You have 12 mL of cells you need to treat with Hydrogen Peroxide (H2O2), such that the final concentration of H2O2 is 50 uM. How much of a 30 mM stock solution of H2O2 would you add to your cells?
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- You have a 1000X stock solution of sodium chloride (NaCl). The concentration of NaCl in the stock solution is 5M. a)How would you make 300mL of a 1X solution? b)What is the concentration of NaCl in your 1X solution?A pharmacist prepares 80 mL of an isotonic solution of 1.1 %w/v Lidocaine HCl (E-value 0.2). To prepare the solution, he dissolves Lidocaine HCl in purified water to make an isotonic solution. Then he dilutes the Lidocaine HCl solution with 0.9%w/v sterile sodium chloride to complete the formulation. How many milliliters of purified water is needed to make the formulation by this method?You have 10 mg/ml ethidium bromide solution. If you add 5 µl of this to 50 ml of agarose gel solution, what will be the final concentration of ethidium bromide in the gel solution in mg/ml?
- How many grams of glucose would you need to make 500 mL of an 8% solution?You receive an order for 750 mL of 27% dextrose solution. You have available D50W, 1000 mL. How many mL of D50W and sterile water are needed to complete the order? *A solution contains 1.0 g of ephedrine sulfate in a volume of 100 mL. What quantity of sodium chloride must be added to make the solution isotonic? E value for the drug is 0.23.
- Using absorbance readings and a standard curve relating absorbance to cell number, you suspect that a bacterial culture contains 7.4 x 109 CFUs/ml. What dilution of this sample would give you a countable plate?How do you calculate the amount of ammonium sulfate necessary to get a 20% solution?Cyclophosphamide, after reconstitution, has a concentration of 20 mg/mL. The patient, who weighs 67 kg and is 155 cm tall, is ordered cyclophosphamide in 250 mL NS. How many mL will be added to the bag?
- We’re back in the lab having fun! Our current experiment calls for us to treat our cells with THC (yeet!, delta 8 from hemp of course lol) and the final concentration of 15 µM once it has been added to the cells. We need to treat 10 mL of cells, and we don't want our treatment volume to be more than 10 µL per 1 mL of cells. What concentration should we make our stock solution? (I submitted this question before and got an answer of 150 µM and that was incorrect)Given 10 gram of vitamin C (E=0.18), we can make ml of isotonic Vitamin C solution.A 1.143 g sample contains only vitamin C (C,H, 0,) and sucralose (C,H,Cl, O,). When the sample is dissolved in water to a total volume of 31.7 mL, the osmotic pressure of the solution is 3.91 atm at 285 K. What is the mass percent of vitamin C and sucralose in the sample? vitamin C: sucralose: %