What is the Rf value of each amino acid observed?
Q: Question 11: The ESI MS spectrum of a protein biomarker of a disease shows 3 consecutive peaks at m/...
A: Mass spectrometer has three important parts, an ionization source (to create atomic and molecular io...
Q: a. You selectively label phospholipids with a fluorescent dye and perform the FRAP assay. You detect...
A: FRAP is a method to determine the diffusion or kinetics of the biomolecule through tissue or cells. ...
Q: Which subunit of bacterial RNA polymerase interacts with the -10 region of the promoter?
A: There are two questions on Transcription.
Q: In First order, the rate of reaction would be . ., if the concertation of an enzyme is increased by ...
A: The reaction may be classified according to the order of reaction, which is the number of reacting s...
Q: Chlorophyll can only be found in plant cells. True or False
A: Photosynthesis is carried out by the plant, which is a multicellular eukaryotic organism. Photosynth...
Q: If a given double stranded DNA undergoes enzymatic hydrolysis targeting only the "a" side in the pho...
A: Here in question, I believe “a” side in Phosphodiester bond refer to the side bond to 3’-C atom of t...
Q: Which of the following statements is true of double stranded DNA? The chains have a backbone of l...
A: Double helix (double stranded helix) is the structure of a DNA molecule.
Q: What determines the characteristics of an amino acid?
A: Amino acids are organic compounds with two functional group namely amino and carboxyl. There are 20...
Q: Consider two enzymes catalyzing two reactions (A --> B --> C ) in a metabolic cascade with their pro...
A: Ans) The order of concentration of A, B, C are in order [C] > [B] > [A] Explanation: The abov...
Q: What are the general effects of stimulants and the specific effects of caffeine, nicotine, amphetami...
A: Stimulants: Stimulants are drugs that fasten up the central nervous system. It causes increased hea...
Q: 3. Tumor derived growth factor B (TGFB) is a secreted protein that has a diverse range of blological...
A: TGF-β (Transforming Growth Factor-β) employs the Smad proteins as the intracellular mediator of sign...
Q: 1. What are the numbers of carbon atoms derived from the first acctyl-CoA that binds to fatty acid s...
A: Fatty acid synthase complex bind to Acetyl-CoA and other substrate and combine the C-atom backbone i...
Q: How does one measure Km using a Lineweaver-Burk plot? 1. By determining the reciprocal of the y-int...
A: Lineweaver-Burk plot is also known as double reciprocal plot suggested by Hans Lineweaver and Dean B...
Q: 1mL of the stock solution acetaminophen (1mg/mL) is used for the estimation of PC using n-octanol. A...
A: Acetaminophen is also commonly known as a Paracetamol that is a medicine used to treat the fever and...
Q: Answer the following: This type of lipid is a complex mixture of esters of long-chain carboxylic ac...
A: Biomolecules are organic molecules made up of mainly carbon and hydrogen but there are other element...
Q: Examine the biochemical composition of a human body in the perspective of the various organic molecu...
A: The human body is composed of not only bones, organs, glands, and tissues but thousands of different...
Q: Some bacteria produce the enzyme penicillinase, which breaks the beta-lactam ring in the penicillin ...
A: Penicillin is able to kill bacteria by inhibiting their cell wall biosynthesis . The cell walls of b...
Q: Why are polyunsaturated omega-3 fatty acids considered as good for our health? Explain such phenomen...
A: Fatty acids are important components of lipids. Fatty acids are composed of hydrocarbon chain and a ...
Q: Why do we compute for TFM in soaps. How does one calculate for TFM? What is saponification? Show it...
A: 1. The TFM quantity interprets the quality of soap. It adds moisturizing properties to the product, ...
Q: An enzyme E binds a substrate S and a cofactor C. The equilibrium dissociation constant Kd,S of the ...
A: Enzymes are usually made of protein molecules which increase the rate of biochemical reaction by dec...
Q: Which of the following are true about aspirin
A: The question is all about the drug aspirin i.e acetylsalicylic acid that is used to reduce pain and ...
Q: Choose (+) if the following circumstances or situations will most likely result to production of int...
A: Hi! Thank you for the question. We are authorized to answer three to four subparts at a time, since ...
Q: Starting from formaldehyde, ammonia, hydrogen cyanide, carbon dioxide, ethene and water as needed, s...
A: 4.5 billion years ago our planate originate from the solar system. at 1st billion years...
Q: what is the importance of getting retention factor (rf) value in chromotography tools?
A: Rf also known as retention factor is used in thin layer chromatography (TLC) and high performance li...
Q: 2) A group of three researchers (who often tend to disagree with one another) isolated a new protein...
A: a) This must be enzyme containing a iron- sulphur cluster not a Mn- cluster.Iron- sulphur cluster co...
Q: Give the functions of the following ingredients, then name a branded/commercial skin or hair care pr...
A: Ozokerite wax is dark brown or light yellow color mineral wax. It is made up of carbon and h...
Q: All of the following are true of the lymphatic system, EXCEPT: which option below is the answer? N...
A: Lymphatic system: It is a system of thin tubes that runs through the body called the lymph vessels....
Q: To which class of enzymes does each of the following belong?
A: Enzyme classification in needed to name the enzymes. According to the Enzyme Commission there are si...
Q: Order the following choices to indicate the sequence of steps in the process of elongation of a grow...
A: The process of translation involves the synthesis of polypeptide chain and proceeds in three stages:...
Q: movement of water into the hypertonic solution. If a cell is hypotonic relative to its environment, ...
A: Biological transport of solute and solvents often occurs via semipermeable membranes. Such a mode of...
Q: The released energy obtained by oxidation of glucose in cell respiration is transient stored as . O ...
A: All biological reactions that take place in a cell are referred to as the metabolism. It is divided ...
Q: A solution has a hydrogen ion concentration of 0.01 mol/L. What is its pH? What is its hydroxide ion...
A: Given Values: [H+] = 0.01 Mol/L = 1×10-2 M pH is a measure of negative logarithm of hydrogen ion con...
Q: What risks are involved in genetic engineering of crop plants? How do these risks compare with other...
A: Genetic engineering is a technique where the genes are edited or newly genes are inserted using gene...
Q: Compound A below is a key intermediate in the synthesis of keto-myo-inositol B. Suggest a synthetic ...
A: Here compound C is D-Glucose D-Glucose is converted into Glucose-6-Phosphate in presence of Hexokina...
Q: What enzyme kinetic parameters are apparantly impacted by uncompetitive inhibitors? Vmax ...
A: In UNCOMPETITIVE INHIBITION, an inhibitor bind at a site distict from the substrate. However, uncom...
Q: A) illustrate in molecular detail how hemoglobin's reduced oxygen affinity is caused by protonation ...
A: The significance of histidine in hemoglobin structure is the binding of oxygen. The histidine forms...
Q: Make a schematic diagram for the synthesis, storage, release, and degradation of acetylcholine.
A: Acetylcholine is main neurotransmitter of the parasympathetic nervous system.
Q: When a protein denatures in a cell, it tends to assemble into aggregates. Propose an explanation for...
A: Proteins have distinct structures. If they are exposed to specific conditions, they may lose their s...
Q: Q7. Kinetics were analysed for an enzyme in the absence and presence of inhibitor. In the absence of...
A: In competitive inhibition the inhibitor competes with substrate for binding to an active site. In no...
Q: 1 0.8 0.6 Mutant y 0.4 Wild-Type 0.2 40 60 80 [ligand] 20
A: According to the given information, the wild type protein structure has aspartate in its primary str...
Q: What will be the color of saliva extraction with iodine in 3, 6 9, 12, 15, and 18 mins if: you put 5...
A: Carbohydrates are divided into 3 classes monosaccharide, disaccharide, and polysacchari...
Q: Describe the mechanism of action of salivary amylase. Explain how it works and on what type of molec...
A: Carbohydrate along with protein, fat and water comprise macro-molecules which are required in larger...
Q: A hypothetical electron-transport chain contains an electron donor (1), an electron carrier (2), and...
A: All biological reactions that take place in a cell are referred to as the metabolism. It is divided ...
Q: QUESTION 9 What are the various ways of dispensing heparin in a biomaterial? Compare and contrast.
A: Heparin is a linear polysaccharide synthesized from proteoglycan. Heparin is made up of repeating di...
Q: What name is given to the assembly of proteins that carry out DNA copying at a replication fork? tra...
A: “Since you have asked multiple questions, we will solve the first question for you. If you want any ...
Q: Thymidine is the result of Removal of the ribose-5'-phosphate group from thymidylic acid Removal of ...
A: Thymidine, also known as deoxythymidine, is a nucleoside which is present in deoxyribonucleic acid. ...
Q: Complete the following paragraph describing the fate of the second 14CO-acetyl-CoA molecule incorpor...
A: The glyoxylate produced in the isocitrate reaction combines with 2nd molecule of acetyl coa in the m...
Q: Using 25 μL of serum, how much diluent would be needed to create a 1:2 dilution? ______ μL
A: The process of reducing a solute concentration by adding solvent to a solution is referred to as dil...
Q: Describe the progression of this meal through the digestive system beginning in the mouth and ending...
A: Ingestion Food processing begins with ingestion (eating). The teeth aid in mechanical digestion by...
Q: How the amino acid tryptophan can be synthesized ?
A: Biomolecules are organic molecules made up of mainly carbon and hydrogen but there are other element...
What is the Rf value of each amino acid observed?
Trending now
This is a popular solution!
Step by step
Solved in 5 steps with 4 images
- The standard curve to determine the amount of betacyanin is shown below. You extracted a red pigment from a beet disc (the mass of a disc is 2 g) using 10 ml of 20% ethanol solution. You measured absorbance of the solution above the beet disc every minute for a total time of 20 minutes. The increase in absorbance was linear during a period of time from 1 min to 10 min. The absorbance at 10 min was 0.8. Calculate the amount of betacyanin extracted from 1g of a beet tissue per minute. Explain your calculations. You can use Excel or a calculator.The calibration curve shown below was used to analyze an unknown protein solution. What is the concentration of the unknown solution, if the absorbance of the unknown is 0.505? Answer in ug/mL. 1.2 y%3D0.005x+0.061 R=0.992 0.8 0.6 0.4 0.2 50 100 150 200 250 Concentration ug/ml Absorbancea) What are the methods used for cholesterol determination? Explain the basis of the method you used in your experiment by writing.b) In the experiment conducted for the determination of cholesterol, the function of the calibration curve obtained using standard cholesterol solutions of 25-400mg/dl was determined as y= 0.0009x.Since the absorbance of the serum sample was determined as 0.28, how many mg/L is the amount of cholesterol in the sample
- You assay 0.5 ml of 1/2000 diluted egg white for protein, as described in the practical schedule, and the spectrophotometer reading at 595 nm is 0.6. Using the attached calibration curve(click to enlarge), calculate the concentration of protein in undiluted egg white. Give your answer in mg/ml.The reults for the macroscopic part: 0.30M glycerin – solution was translucent (could see text behind the test tube) 0.15M NaCl – solution was opaque (could not see text behind the test tube) 0.30M NaCl – solution was opaque (could not see text behind the test tube) 0.15M glucose – solution was translucent (could see text behind the test tube) 0.30M glucose – solution was opaque (could not see text behind the test tube) 0.30M Urea – solution was translucent (could see text behind the test tube) Results for microscopic part: 0.30M glycerin – no cells present 0.15M NaCl – normal sized cells 0.30M NaCl – crenated (shrunken and star-shaped) cells 0.15M glucose – no cells present 0.30M glucose – normal sized cells 0.30M Urea – no cells present Determine the osmolarity (hypoosmotic, isosmotic, or hyperosmotic) and tonicity (hypotonic, isotonic, hypertonic) of the following solutions.In which solutions did the osmolarity NOT match the tonicity? For those solutions, why did the osmolarity…Answer the following questions about protein concentration determination. a) At what wavelength should an albumin protein solution placed in a quartz cuvette be measured in the spectrophotometer? Why is that? b) With which methods other than spectrophotometer can the amount of protein be measured quantitatively?
- Five amino acids were subjected to reverse phase chromatography, and the following Rf values were computed: A - 0.23 B - 0.08 C - 0.54 D - 0.74 E - 0.96 Which is the most polar amino acid?Five amino acids were subjected to normal phase chromatography, and the following Rf values were computed: A - 0.59 B - 0.12 C - 0.71 D - 0.33 E - 0.20 Which is the most polar amino acid?Four known amino acids (AA) produce a chromatograph with Rf values as follows: AA1 =0.25, AA2 =0.66, AA3=0.50,AA4=0.80. A mixture of three amino acids yields spots that are 2 cm,5.2cm, and 6.4 cm from the organ. The solvent front is 8.0 cm from the origin. Which three of the four amino acids are contained in the mixture?
- A 2.5mg/ml solution of ampicillin is available for use, what volume would youadd to 200ml of LB medium to obtain a final concentration of 50µg/ml ofampicillin?(b) Table Q1 below provides optical measurements of samples with known concentrations of analyte (ten samples were measured and the average and standard deviation are presented). They are also represented graphically in Figure Q1, below the table. Analyte concentration (nM) 0.1 0.2 0.5 1 2 5 10 15 fluorescence intensity 300000 250000 200000 150000 100000 50000 0 0 Table Q1 Fluorescence intensity (average) 9300 9200 10800 19600 37200 90000 178000 224000 5 [Analyte]/nM 10 Standard deviation 2000 1200 1000 2000 10000 30000 25000 40000 15 Figure Q1. Signal intensity for different analyte concentrations. Error bars are standard deviation. (ii) Calculate the limit of detection (LOD) for this sensor. (iii) A patient sample measured using this biosensor in the diagnostic laboratory generates a signal of 142800. The coefficient of variation of the device is 5%. Explain whether the diagnostic lab would have the confidence to report that the sample has a concentration in analyte of 8 nM or…1.0 ml of serum albumin (BSA) solution was precisely diluted to 100 ml with a buffer solution, and the absorbance at 280 nm was measured from this buffer solution at a distance of 1 cm from the light. The result was A=1.0. BSA is 0.1%(=0.1g/l). The absorptivity is e(0.1%)=0.667, so 1g/l solution gives an absorbance of 0.667. What was the original protein content? Report in the result unit mg/ml with an accuracy of 0.1 mg.