What is the Rf value of each amino acid observed?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
What is the Rf value of each amino acid observed?

Transcribed Image Text:A mixture of amino acids was subjected to paper
chromatography with six amino acid standards in order
to determine its composition. After visualization using
ninhydrin, the measured distances were as follows:
Distances:
F
Solvent - 10.3cm
X
A - 4.5cm
B - 1.0cm
A
C - 6.6cm
D
E
D - 3.9cm
E - 3.5cm
B
F - 9.1cm
G - 10.3cm
X1 - 0.7cm
X2 - 4.3cm
X3 - 7.1cm
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