As an intermediate step in making the enzyme solution, you need to prepare Buffer X using the stock solutions of each chemical. Describe how you would make 100 mL of buffer X that has the following concentrations and pH: 50mM NaCl, 20mM Tris, 5mM MgCl2, pH 7.5 You do this starting with stock solutions of 1M NaCl, 1M Tris, 1M MgCl2, and water and a pH meter.
As an intermediate step in making the enzyme solution, you need to prepare Buffer X using the stock solutions of each chemical. Describe how you would make 100 mL of buffer X that has the following concentrations and pH:
50mM NaCl, 20mM Tris, 5mM MgCl2, pH 7.5
You do this starting with stock solutions of 1M NaCl, 1M Tris, 1M MgCl2, and
water and a pH meter.
Calculations play an important role in taking the right concentration of solutes. They help to determine the amount of solution required to make a solution of a particular concentration.
Solutions prepared at the correct concentration helps to obtain the correct result for the experiments. The reproducibility of the experiment is mainly determined by the correct amount of solute or solution taken for the reagent preparation.
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