Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
[2] Calculate the pH of the arginine solution after the addition of Oml, 5ml of 0.1M NaOH and calculate pH after addition of 0.5 ml, 2 ml of HCI

[2] Calculate the pH of the arginine solution after the addition of Oml, 5ml of 0.1M NaOH and calculate pH after addition of 0.5 ml, 2 ml of HCI

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question
#2
**Method:** 1. **Pipette Preparation:** - Pipette 10 ml of alanine solution (0.1 M) into a 50 ml beaker. 2. **Acid Addition:** - Add 0.5 ml of (0.1 M) HCl from the burette and determine the pH of the solution after each addition. - Continue adding acid until the pH falls to about 1.3. 3. **Base Addition:** - Wash the electrode in distilled water, then titrate a further 10 ml of alanine solution with 0.1 M NaOH until pH reaches 12.5. 4. **Plotting:** - Plot a titration curve for alanine (pH versus titrant in ml). 5. **Arginine Titration:** - Perform the titration of arginine in a similar way and plot their titration curves. **Result:** - Record the titration table and plot a curve of pH versus ml of NaOH and HCl added. - Determine the pKa and pI values from your curves and compare them with the standard values. - **For alanine:** - pK₁ = 2.34 - pK₂ = 9.69 - pI = 6.01 - **For arginine:** - pK₁ = 2.17 - pK₂ = 9.04 - pK₃ = 12.48 - pI = 10.76 **Discussion:** 1. **pH Calculation:** - Calculate the pH of the alanine solution after the addition of 0 ml, 5 ml of 0.1 M NaOH and calculate pH after addition of 1 ml, 2.5 ml of HCl. 2. **pH Calculation for Arginine:** - Calculate the pH of the arginine solution after the addition of 0 ml, 5 ml of 0.1 M NaOH and calculate pH after addition of 0.5 ml, 2 ml of HCl. 3. **Comparison:** - Compare your calculated pH values with those obtained from the curve. 4. **Buffering Behavior:** - At what pH range did the
Transcribed Image Text:**Method:** 1. **Pipette Preparation:** - Pipette 10 ml of alanine solution (0.1 M) into a 50 ml beaker. 2. **Acid Addition:** - Add 0.5 ml of (0.1 M) HCl from the burette and determine the pH of the solution after each addition. - Continue adding acid until the pH falls to about 1.3. 3. **Base Addition:** - Wash the electrode in distilled water, then titrate a further 10 ml of alanine solution with 0.1 M NaOH until pH reaches 12.5. 4. **Plotting:** - Plot a titration curve for alanine (pH versus titrant in ml). 5. **Arginine Titration:** - Perform the titration of arginine in a similar way and plot their titration curves. **Result:** - Record the titration table and plot a curve of pH versus ml of NaOH and HCl added. - Determine the pKa and pI values from your curves and compare them with the standard values. - **For alanine:** - pK₁ = 2.34 - pK₂ = 9.69 - pI = 6.01 - **For arginine:** - pK₁ = 2.17 - pK₂ = 9.04 - pK₃ = 12.48 - pI = 10.76 **Discussion:** 1. **pH Calculation:** - Calculate the pH of the alanine solution after the addition of 0 ml, 5 ml of 0.1 M NaOH and calculate pH after addition of 1 ml, 2.5 ml of HCl. 2. **pH Calculation for Arginine:** - Calculate the pH of the arginine solution after the addition of 0 ml, 5 ml of 0.1 M NaOH and calculate pH after addition of 0.5 ml, 2 ml of HCl. 3. **Comparison:** - Compare your calculated pH values with those obtained from the curve. 4. **Buffering Behavior:** - At what pH range did the
Expert Solution
Step 1: Amino acid titration

A method employed in laboratories for calculating an amino acid's pKa values and isoelectric point (pI) is known as amino acid titration. This procedure involves titrating an amino acid solution with known concentrations of an acid or a base while continuously checking the pH of the solution.

steps

Step by step

Solved in 4 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS