The graph below is a standard curve generated by plotting the distance travelled by the size standards on an SDS-PAGE gel. If a protein band moved to a distance of 2 cm, the approximate Mol. Wt. of that protein is 17 KDa 70 KDa Not enough information 100 KDa

Q: Suppose you calculate that you can load 2000ng of Sample A and suppose your concentration of Sample…

A: * We must figure out how much Sample B is needed to give 2000ng of protein in order to compute the…

Q: Below is a trace from an analytical gel-filtration column upon which a single sample containing a…

A: Gel filtration column is used in gel filtration chromatography (GFC) technique. GFC is also known as…

Q: During SDS-PAGE,  the charge on glycine in the separating gel is ____________ while the charge on…

A: SDS-PAGE, or sodium dodecyl sulfate polyacrylamide gel electrophoresis, is a technique used to…

Q: 3. Given the partial sequence of a peptide: ILWANRMSHVLFAVE A Which amino acid residues would you…

A: We are authorized to answer one question at a time since you have not mentioned which question you…

Q: A mixture of proteins contains Trypsinogen (24 kDa), fumarase (49 kDa), Transferrin (80 kDa) and…

A: Chromatography is a form of an analytical method that breaks down a chemical mixture into its…

Q: if you had a protein sample solution of unknown concentration which gave an absorbance of 0.992…

A: Answer:- The standard curve gives the absorption value of aliquots having the substance of known…

Q: 2 a. You are trying to purify protein C from a mixture of proteins noted in the above Table. If you…

A: Chromatography is biochemical separation method for organic molecules or solutes of a compound…

Q: 2 L of 5 mg/mL gelatin solution from 1 g/mL gelatin solution

A: Gelatin is a food ingredient that is derived from collagen. It is basically a protein in nature.

Q: During SDS-PAGE,  the charge on glycine in the stacking gel is ____________ while the charge on the…

A: SDS-PAGE is used to separate proteins based upon the relative mobility of charged species. Proteins…

Q: 1 2 3 4 5 6 N Sample Lysate Name volume 8 Tilapia Cod Sole Perch Tilapia Cod Sole 2 μl Perch 10 με 2…

A: Protein sample: A non-branching sequence of amino acids joined together by a single peptide bond…

Q: Research Center received a protein sample in a liquid form from her colleague recently. However, the…

A:

Q: How many copies of a protein need to be present in a cell in order for it to be visible as a band on…

A: * Given that you can load 100 µg of cell extract onto a gel and can detect 10 ng in a single band by…

Q: Describe and explain sources of errors in this experiment. Provide practical suggestions for…

A: Protein solubility refers to the ability of a protein to dissolve in a solvent such as water, and is…

Q: 1. Prepare stock solutions of the 3 proteins in 0.8 mM Phosphate buffer pH=7.40 10 μΜ Conc.A 25 µM…

A: Here Molar Concentration, Molecular weight and Volume of stock solution is given Molar concentration…

Q: In size exclusion chromatography, which component will elute last in the set up?   Group of answer…

A: Size exclusion chromatography is also known as gel filtration chromatography (GFC) or gel permeation…

Q: Given the information:   Tube 6 contains 8.0 g/dL Tube 6 contains 0.5 mL of the dilute standard…

A: The measurement and computation of protein concentrations in a laboratory context are the subject at…

Q: In SDS page, the ________ proteins will move through the gel fastest and first, and in size…

A: SDS-PAGE is biochemistry technique used to separate proteins based upon the relative mobility of…

Q: Two dimensional electrophoresis separates proteins according to their isoelectric focusing and mass.…

A: Two dimensional electrophoresis:- powerful electrophoretic method for separation and analysis of…

Q: 50 microliters of your original sample to the Bradford reagent. What is the concentration of your…

A: Proteins are made up of amino acids that have been polymerized. As a result, amino acids are…

Q: You are developing a porous membrane for use in a dialysis system. The membrane must be able to…

A: Explanation: Detailed explanation: The difference in pressure that exists between the inlet and…

Q: When combining 1ml of lysosome solution with a concentration of 5mg/ml and 1ml of water what is the…

A: Tiny, simple sack-like structures present in cytoplasm of cells are lysosomes. These are also known…

Q: Suppose you have a solute, MW = 64 KDa, in plasma. (Answer with the word "increases" or "decreases"…

A: Diffusion is a process of movement of the solute from the region of high concentration to the region…

Q: average membrane pore radius for this membrane using this information.

A: Given, Average radii=1nm Conc. of filtrate = 2.17 g/L Conc. of feed-side surface = 8.52 g/L Mass…

Q: the optical density of your cell culture is 0.92. How much of this  culture should you add to 500 mL…

A: Optical density (OD), also known as absorbance, is a measure of how much a substance absorbs light…

Q: How should you prepare your running buffer?

A: SDS-PAGE is method of separation of protein based on its size. Running buffer of the SDS-PAGE…

Q: A new protein namely protein X has been isolated by molecular biologists. The protein is shown to…

A: Protein:  Proteins are necessary nutrients for human health. They are a component of body tissue and…

Q: The approximate thickness of the phospholipid bilayer is 10A 1 micro M 50A 150A 1nM

A: There are four classes of biological macromolecules - nucleic acid, proteins, lipids and…

Q: QUESTION 26 If you are required to add 25 ng (nanograms) of a solid into 50 uL (microliters) of…

A: 1 ng = 0.001 microgram (mg)