In SDS page, the ________ proteins will move through the gel fastest and first, and in size exclusion chromatography, the _________ proteins will elute from the column first.     smallest; largest     largest; largest     largest; smallest     smallest; smallest   The volume of the beads is included in (Select all that applies)     VT     Vo     Vi     VS     VL

Q: You are growing yeast cells in your lab. The Yeast Growth Medium contains 56 mM glucose (Mol. Weight…

A: Yeast growth medium, also referred to as yeast culture medium or yeast nutrient medium, is a…

Q: SDS-PAGE. FRAP. thin-layer chromatography. Western blotting. O freeze-fracture microscopy.

A: Protein purification is important for the characterization of the function, structure and…

Q: tion chromatography separates chemicals base on their differences in

A: Chromatography is a technique that is used for the separation of various mixtures.  Depending upon…

Q: Which technique would be the best for analyzing lipid composition of a membrane? freeze-fracturing…

A: TLC- Chemical and immunological stains are used to find the lipids on the membrane. With the help of…

Q: Beginning with 10 grams of plant tissue, order the following procedures (as steps 1-4) that you will…

A: Cell fractionation is a process that involves rupturing cells, separating and suspending cellular…

Q: True or false Explain your answer To observe the Bacterial species E. coli with bright field…

A: Bright field microscopy is a widely used technique in which specimens are observed under normal or…

Q: Solution Stock solution Working solution Volume needed restriction digestion buffer 10X DNA 200…

A: In order to set up restriction digestion, we must mix precise amounts of various components to get…

Q: How many amino acids make up the unknown protein? How many nucleotides in the mRNA transcript? How…

A: Proteins are polymers of amino acids linked by peptide/amide bonds by the condensation reaction…

Q: e fluorescent label in this image is he cell shapes are imaged with The microscopy used for…

A: In the image given some kind of cells are present which are glowing.  Some cellular processes are…

Q: When combining 1ml of lysosome solution with a concentration of 5mg/ml and 1ml of water what is the…

A: Tiny, simple sack-like structures present in cytoplasm of cells are lysosomes. These are also known…

Q: If 10 µL of cells in suspension was mixed with 20 µL of a dye called trypan blue. DF = _____.…

A: According to the question, If 10 µL of cells in suspension was mixed with 20 µL of a dye called…

Q: 8.if you collect a swab of cells from the inside of your mouth and perform the Gram stain technique,…

A: The gram staining is a differential stain that utilizes the difference in the cell membrane and cell…

Q: 10mL of 1x solution from a 2x stock solution.

A: Dilution formula - C1V1 = C2V2 C1 = concentration of stock C2 = final concentration V1 = volume of…

Q: Negatively charged proteins migrate towards the top of the gel backwards towards the positive…

A: The gel electrophoresis is a technique by which different biological samples including DNA, RNA and…

Q: Protein Concentration Absorbance at 595nm (mg/mL) 0.05 0.0092 0.1 0.2 0.02 0.04 0.3 0.112 0.167 0.4…

A: There are few important points : To determine the amount of protein in an unknown sample is termed…

Q: Standard curves You are setting up a standard curve for lysozyme. You have a stock vial containing 2…

A: Proteins are chains of amino acids bonded via amide bonds. Lysozyme is an antimicrobial enzyme that…

Q: Short Essay: Answer the prompt in 4 sentances or less. Describe the staining technique used to…

A: Staining is a protocol used to visualize cells/ tissues under a microscope. Staining colors and…

Q: The technique used to determine protein type or kind of protein in a membrane is called (type…

A: Proteins are macromolecules which are build up of long chain of amino acids joined together with…

Q: True or false?       light microscopy has a limit of resolution of 200nm.   phase contrast…

A: Microscope is an instrument used to visualize minute / microscopic things . There are various types…

Q: Table 5-2: CDNB Assay Absorbance Values (at 340 nm) for Anion Exchange Fractions IEX FT Fraction…

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Q: Gastroenteritis may be caused by microbes belonging to which of the following groups? All of the…

A: These are some questions regarding microorganisms. It is required to select the correct option.

Q: How many cells would you be plating if you pipetted 200uL into your dish from a 125 x 104 cells/mL…

A: The question is of calculation with keeping the units in our mind .

Q: Below is shown the FRAP profiles of three proteins X, Y and Z, which of the protein(s) is/are most…

A: FRAP stands for fluoresence recovery after photobeaching(FRAP) is a technique to study mobility of…