3. A mixture of proteins shown in the table below are dissolved in water and injected into a size exclusion chromatography system. Given the calibration curve for the SEC column, draw a qualitative curve to show the expected results (chromatogram) of running the SEC by showing detection units (RI or UV absorbance) on the y- axis and time on the y-axis. Relative peak heights (areas under curves) and times should be as quantitative as possible. ID 1 2 3 4 5 6 Protein Collagen Lactoperoxidase Insulin Fibronectin Lysozyme Trypsin Molecular Weight 68,000 92,600 5,800 55,000 16,000 47,000 Note: as shown in the calibration curve, the beads in the column can separate molecules less than ~120,000 molecular weight, but any proteins larger than that will elute at ~5 minutes. The minimum molecular weight that can be separated in the column is is 20,500; smaller proteins elute at 25 minutes. The equation of the calibration curve is shown on the graph. Conc pl 0.5 g/L 1 g/L 12 g/L 5 g/L 2 g/L 10 g/L log(Molecular Weight) 8 65432 7.8 6.5 Type fibrous a-helices; structural antibacterial enzyme globular protein fibrous protein 7.3 4.6 4.6 cell-lysing enzyme 2.5 digestive enzyme Calibration Curve for SEC Column 3log (MW) = 5.262 -0.0378t R² = 0.9991 10 20 Elution Time, min 30
3. A mixture of proteins shown in the table below are dissolved in water and injected into a size exclusion chromatography system. Given the calibration curve for the SEC column, draw a qualitative curve to show the expected results (chromatogram) of running the SEC by showing detection units (RI or UV absorbance) on the y- axis and time on the y-axis. Relative peak heights (areas under curves) and times should be as quantitative as possible. ID 1 2 3 4 5 6 Protein Collagen Lactoperoxidase Insulin Fibronectin Lysozyme Trypsin Molecular Weight 68,000 92,600 5,800 55,000 16,000 47,000 Note: as shown in the calibration curve, the beads in the column can separate molecules less than ~120,000 molecular weight, but any proteins larger than that will elute at ~5 minutes. The minimum molecular weight that can be separated in the column is is 20,500; smaller proteins elute at 25 minutes. The equation of the calibration curve is shown on the graph. Conc pl 0.5 g/L 1 g/L 12 g/L 5 g/L 2 g/L 10 g/L log(Molecular Weight) 8 65432 7.8 6.5 Type fibrous a-helices; structural antibacterial enzyme globular protein fibrous protein 7.3 4.6 4.6 cell-lysing enzyme 2.5 digestive enzyme Calibration Curve for SEC Column 3log (MW) = 5.262 -0.0378t R² = 0.9991 10 20 Elution Time, min 30
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
A qualitative graph!
Transcribed Image Text:3. A mixture of proteins shown in the table below are dissolved in water and injected into a size exclusion
chromatography system. Given the calibration curve for the SEC column, draw a qualitative curve to show the
expected results (chromatogram) of running the SEC by showing detection units (RI or UV absorbance) on the y-
axis and time on the y-axis. Relative peak heights (areas under curves) and times should be as quantitative as
possible.
ID
1
2
3
4
5
6
Protein
Collagen
Lactoperoxidase
Insulin
Fibronectin
Lysozyme
Trypsin
Molecular Weight
68,000
92,600
5,800
55,000
16,000
47,000
Note: as shown in the calibration curve, the beads in the
column can separate molecules less than ~120,000
molecular weight, but any proteins larger than that will
elute at ~5 minutes. The minimum molecular weight that
can be separated in the column is is 20,500; smaller
proteins elute at 25 minutes. The equation of the
calibration curve is shown on the graph.
Conc
0.5 g/L
1 g/L
12 g/L
5 g/L
2 g/L
10 g/L
log(Molecular Weight)
8765432
pl
7.8
6.5
0
7.3
4.6
4.6
2.5
Type
fibrous a-helices; structural
antibacterial enzyme
globular protein
fibrous protein
cell-lysing enzyme
digestive enzyme
Calibration Curve for SEC Column
3log (MW) = 5.262 -0.0378t
R² = 0.9991
10
20
Elution Time, min
30
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 3 steps with 1 images
Recommended textbooks for you
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON