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Q: Which would you choose if you needed to make a partial diploid for complementation analysis? rolling…

A: The correct answer is option 1. Rolling circle plasmid.

Q: Use the figure of the gel below to answer the following questions. Controls 1 2 3 Experimentals 4 5…

A: Gel electrophoresis is a technique used to separate DNA fragments,( or may be RNA and protein) based…

Q: PCR analysis of your sample did not work can you suggest why? How can you test if this is true?

A: PCR  stands for polymerase chain reaction    *PCR is nowdays used often in  hospitals and large…

Q: In an experiment a student’s sample amplified for D1S80 produced 3 bands. It was the only DNA sample…

A: PCR  amplified sample is run on gel electrophoresis for the detection of  results and  comparing it…

Q: You are counting plaques on your plaque assay plates made from serial dilutions of your high titer…

A: A serial dilution is any dilution in which the concentration decreases by the same dilutions in each…

Q: Comment on the failure of p1vir transduction experiments and give a reason why you think the…

A: transduction experiments is a technique commonly used in molecular biology to introduce foreign DNA…

Q: How would I know if my PCR product is pure?

A: Polymerase Chain Reaction (PCR) is one of the most significant tools of molecular biology. It was…

Q: Which of the options listed below can serve to carry a gene from one organism into a bacteria cell?…

A: The specific genes or pieces of DNA are transported or carried from one organism to another. These…

Q: Jm109 cells transformed with ligation and plasmid alone is used. The colonies most like:   white…

A: β-galactosidase is an enzyme encoded by the lacZ gene and is used to break down lactose to glucose…

Q: the answer I was told was that the gap would remain in newly sythesized dna from lagging strand. How…

A: The main function of DNA ligase is to seal the gap that occurs in the lagging stand formation.…

Q: What is the role of the following in the alkaline plasmid screen?    G buffer (Cell Suspension…

A: G buffer (Cell suspension solution): It is a solution that consists of a mixture of Tris (pH 7.5)…

Q: After adding dna ligase to plasmid alone the sample could serve as:   experimental sample…

A: Plasmids are most commonly found as small circular, double-stranded DNA molecules in bacteria. It is…

Q: Questions 11-14. Melia and Sophie performed a PCR experiment using DNA purified from their phage…

A: * PCR is the polymerase chain reaction is a molecular techniques that forms invitro conditions. * It…

Q: Pcr primers are:  single strand 15-25 bases long incorporated into the newly synthesized DNA…

A: PCR stands for polymerase chain reaction. It is one of the technique of biotechnology.

Q: A pcr required 5 ul of water To make master for 5 samples you would add   35 ul of water   30…

A: PCR stands for polymerase chain reaction. It is used to synthesize the specific region of DNA.

Q: Jerome Vinograd found that when circular DNA from apolyoma virus is subjected to cesium chloride…

A: Polyoma virus is the virus that infect the human and establish symbiotic relationship with humans to…

Q: Why should plasmid sample be mixed in the inverted position and not vortexed?  Will these actions…

A: Plasmids are small circular pieces of DNA that can be found in many bacteria and are often used in…

Q: put the follwoing steps in chrnological order based upon what you would need to do to deternine if…

A: DNA precipitation or determination of DNA in the organism is a complex process that involves the…

Q: PCR uses a very special DNA polymerase, tell me where it came from and why it is special to PCR.

A: PCR stands for polymerase chain reaction. It is used to amplify the specific region of template DNA.

Q: Select all the statements that provide evidence that DNA is the genetic material. Check All That…

A: The eukaryote chromosome is linear and contains both DNA and protein, and both are found in equal…

Q: Select all the statements that provide evidence that DNA is the genetic material. Check All That…

A: 1 ans... Why bat eukaryotic chromosome contain more protein than DNA because DNA is wrapped on…

Q: Tailed primer can have restriction enzyme sites. True False

A:

Q: Arrange the following steps in the PCR experiment 1 3 5. Add samples and reagents Extend Anneal…

A: (According to our guidelines we are required to answer the first question in case of multiple…