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Need help
To prove a BIM had evolved to be phage 2972 resistant, what experiment could you carry out?
(already carried out 2% agarose gel PCR, what experiment could be carried out that is similar?)
Step by step
Solved in 2 steps
- Comment on the failure of p1vir transduction experiments and give a reason why you think the transductions was unsuccessful for some genes. Suggest any alternative methods that could be used to create the mutations that were unsuccessful in these experiments? There are no colonies on the plate for some of the genes. and in DNA sequence, some colonies don't have primers present. For PCR, some colonies are not replaced by kanamycin or tetracycline.A researcher is doing PCR to have more of HDR template which will be used further for yeast transformation. He column-cleaned the PCR, eluting in 20 ul of warm elution buffer. He used 2 ul of the 20 ul stock to nanodrop the sample and found the concentration was 60 ng/ul. Find the nanograms of HDR template he had left to use for future work (like yeast transformations)?Which of the following experimental techniques was used by Hershey and Chase to establish the genetic material of phage? * None of the above X-ray Isotope labelling Chromatography Ultracentrifugation
- What type of experiment would you be most likely to use to demonstrate that protein X was directly phosphorylated by the M-cyclin/CDK pair a flow cytometry experiment a PCR based assay an in vitro assay a fluorescence microscopy experimentHi, I am working on qPCR quantification for specific bacteria. However, when the CT value achieve greater than 30, it always shown high CT value differences in my duplication no matter I repeat the same set of experiment for those samples with low quality. My question is, what makes this happening? The other question is, how many CT value differences is acceptable for duplication?Looking at the model data i upload and given the question how could i analyse tboth experimental results (antibiotic selection and agarose gel). Including one clearly labelled image of the agarose gel and with an accompanying legend. And how could i interpret the findings of the results and draw a conclusion i.e. what is in each tube? Also is there any further experiments that I could perform to confirm the identity of the plasmid stocks?
- Part A. If student counts 63 colonies on their 10^-6 dilution LB plate. What was the original concentration of their cells if they plated 100ul? Part B.If we used pGFPuv as the template for PCR positive control. This is because: a. it contains the GFP gene so it should show a product. b. It contains DNA fragments that were added to the ligation reaction. c. It is the desired plasmid we wanted to make. d. So we have a band to compare our unknown plasid to allowing us to check if the unknown is the right size.Recent scientific studies show that the plasmid plays a vital role in gene transfer not only in the field of bacteriology but microbiology on the whole. Give a detailed account of how bacterial gene transfer can be carried out as Medical Laboratory Scientist.A amp PBR322 4301 fot B Clear Zones Figure 2 The postgraduate student, Demika, inserted her gene of interest into the plasmid, pBR322, before transformation into the competent host cell using heat shock method. After that she cultured the cells on the Ampicillin agar plate before replica plating the colonies onto another Ampicillin (A) and Tetracycline (B) agar plates shown in Figure 2. (1) Referring to the vector pBR322 in Figure 2, which recognition site was cleaved to insert the gene of interest? Based on the observation above, can you identify which colonies are carrying positive mcombinants of BR322? Explain your selection.
- 3 different pcr tubes were obtained each tube containing a variation of hemoglobin E. tube A is a normal hemoglobin E,tube B is a diseased hemoglobin E, and tube C is a hemoglobin E trait. conduct a graph showing which one would move further electrophoresis gel well if an experiment was performed. explain your answer.About the technique of phage display: MOLECULAR BIOLOGY_advanced The Escherichia coli cell infected by the phage codifies for the optimized ligand when the phage DNA integrates in the DNA of the bacteria. Phages are selected if they express on their surface the optimized ligand. One selects Escherichia coli cells that are resistant to the phage infection. More than one optimized ligand can be selected during the panning procedure. The ligand to be selected on the surface of the phage is non-covalently linked to one of the surface proteins.Describe and contrast the common steps of DNA replication in vivo and the PCR reaction in vitro? In simple terms so, that I can understand. Thank you