Which of the following prokaryotic cells would probably not be “competent” at transformation? an Escherichia coli cell blasted with a “gene-gun” carrying DNA on gold particles an Escherichia coli cell treated with dimethyl-sulfoxide (DMSO) a Streptococcus pneumoniae cell with no special treatments an Escherichia coli cell with no special treatments E. an Escherichia coli cell treated with calcium
Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
Which of the following prokaryotic cells would probably not be “competent” at transformation?
- an Escherichia coli cell blasted with a “gene-gun” carrying DNA on gold particles
- an Escherichia coli cell treated with dimethyl-sulfoxide (DMSO)
- a Streptococcus pneumoniae cell with no special treatments
- an Escherichia coli cell with no special treatments
E. an Escherichia coli cell treated with calcium
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