Fréderick Griffith's discovery of transformation relies principally upon O A. slow renaturation of RNase A by removal of urea with mild oxidation O B. density labeling DNA with the stable isotope 1PN OC. distinction between R and S strains of pneumococcus D. labeling of bacteriophage proteins with 35s
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see the attached multiple choice question. thank you
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- All are true for DNA polymerase EXCEPT: / Almal geld vir DNA-polimerase, BEHALWE: A. generates dsDNA from SSDNA / genereer dsDNA vanaf SSDNA B. requires a primer with a free 5'- OH end, but the 3'-end may be phosphorylated / benodig 'n voorvoerder met 'n vrye 5'-OH-groep, maar die 3'-einde kan gefosforileer word. C. copies the sequence of nucleotides of one strand in a complementary fashion. / kopieer die volgorde van nukleotiede van een streng komplementêr. D. synthesizes new strands by adding successive nucleotides in the 5'→3' direction. / sintetiseer nuwe stringe deur opeenvolgende nukleotiede in die 5 '→ 3' rigting toe te voeg. E. copies the sequence of nucleotides of one strand to form a new second strand. / kopieer die volgorde van nukleotiede van eenH. State if/how Aureliano's mutation changes the amino acid sequence and describe the effect that change has, if any, on the protein.What Art the Features of the Series of -omes? Define the following terms: a. Genome b. Transcriptome c. Proteome d. Metabolome e. Fluxome
- Chemical Mutagenesis of DNA Bases Show the nucleotide sequence changes that might arise in a dsDNA (coding strand segment GCTA) upon mutagenesis with (a) HNO2, (b) bromouracil, and (C) 2-aminopurine.1. Choose the phrase from the right column that best fitsthe term in the left column.a. transformation 1. requires supplements in medium forgrowthb. conjugation 2. a method for mutagenizing genes inbacterial genomesc. transduction 3. small circular DNA molecule that canintegrate into the chromosomed. lytic cycle 4. the core genes that define a bacterialspecies plus all of the genes unique toindividual strainse. lysogeny 5. transfer of DNA requiring directphysical contactf. episome 6. integration of phage DNA into thechromosomeg. auxotroph 7. infection by phages in which lysis ofcells releases new virus particlesh. pangenome 8. transfer of naked DNAi. gene targeting 9. transfer of DNA between bacteria viavirus particlesThe restriction enzyme Haelll has the recognition sequence below and cuts between the G and C bases. 5'...GG'CC...3' 3...CC'GG...5' Determine the number of fragments that would be produced with the following sequence. 5'-ATTGCGCTCAAAGGCCAATCATCAGGTTC-3' 3'-TAACGCGAGTTTCCGGTTAGTAGTCCAAG-5' O 1 O 2 O 3 O 4 MacBook Pr
- Bacterial genomes such as that of E. coli typicallyhave only a single origin of replication, from whichreplication proceeds bidirectionally. Pol III, the DNApolymerase responsible for replicating the E. colichromosome, synthesizes DNA at a rate of about1000 nucleotides per second.a. From this information, estimate the minimum generation time of E. coli.b. Under optimal conditions, E. coli have been observedto divide in as little as 17 minutes. Speculate howthis might be possible, given your answer to part (a).In E. coli, Base excision repair (BER) handles non-bulky lesions (e.g., deamination, oxidation) and nucleotide excision repair (NER) handles bulky lesions (e.g., Py dimers). Is this statement true or false? O True FalseWhich of the strands of DNA could act as a PCR primer for the DNA sequence shown below? 5’-CCCTGGGCTCTGTAAATGTTTCTAAGTG-3’ 3’-GGGACCCGAGACATTTACAAAGATTCAC-5’ A. 3′-ACTGTTAGA-5′ B. 3′-AAATTTGGC-5′ C. 3′-ATGCTTTGA-5′ D. 5′-GGGACCCGA-3′ E. 5′-CCCTGGGCT-3′
- Explain the following statement : a) initiation of bacteriall DNA replication is an energy requiring process b) bacterial DNA polymerase can enter the termination sequence but cannot exisThe anti-viral drug Acyclovir is a nucleotide analog that is lacking the 3’ OH group which is required to form a 3’→5’ phosphodiester bond. This drug is ineffective against DNA polymerases with proofreading abilities, which is why human DNA polymerases are not targeted. Acyclovir can be used to treatsevere cases of Epstein-Barr viral (EBV) infection, but has little to no effect under non-severe infections. Based on this information, EBV will use ________ DNA polymerase during severe infections and __________ DNA polymerase during non-severe infections. Human; Human EBV; Human EBV; EBV Human; EBVA. Avery, MacLeod and McCarty proved DNA is the genetic material. If you were to repeat their experiment and do the following treatments what results would you expect? You can state if the mice will have diseases or no disease Infect Mice with If mixed with Live R-Strain Extract from heat killed S- Strain treated with DNase D. 5' 3' Topoisomerase HHHHHH B. If you were to repeat the experiment done by Hersehy and Chase and decided to use 14C (instead of 35S) and 32P isotopes, which molecules will be labeld in the T2 bacteriophage. Will it prove DNA is the genetic material? C Extract from heat killed S- Strain treated with RNase C. Many enzymes and proteins are involved in DNA replication. List each one of them (from the diagram below and also those not included in the diagram) and briefly describe their function. Label which one will serve as the template for the leading strand and which one will be the template for the lagging strand. IIH Helicase Primase 3' Prediction 5' Replication…