You want to find E. coli genes necessary for lactate metabolism. You have a population of mutagenized E. coli. Which screening protocol would you use to find mutations in genes necessary for lactate metabolism? First plate on lactate media, then replicate plate on galactose media None of the options are correct Look for mutants that constitutively express lactate-metabolizing enzymes Plate directly on lactate media First plate on galactose media, then replicate plate on lactate media

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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You want to find E. coli genes necessary for lactate metabolism. You have a
population of mutagenized E. coli. Which screening protocol would you use
to find mutations in genes necessary for lactate metabolism?
First plate on lactate media, then replicate plate on galactose media
None of the options are correct
Look for mutants that constitutively express lactate-metabolizing enzymes
Plate directly on lactate media
First plate on galactose media, then replicate plate on lactate media
Transcribed Image Text:You want to find E. coli genes necessary for lactate metabolism. You have a population of mutagenized E. coli. Which screening protocol would you use to find mutations in genes necessary for lactate metabolism? First plate on lactate media, then replicate plate on galactose media None of the options are correct Look for mutants that constitutively express lactate-metabolizing enzymes Plate directly on lactate media First plate on galactose media, then replicate plate on lactate media
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