Beadle and Tatum Experiment X rays Conidia from the cultures that fail to grow on minimal medium are then tested on a variety of supplemented media (a) Mutagenized conidia Microscopic ascospores aro dissected and translerred Crossed with wild type one by one to culture tubes Wild type of opposite mating type (d) Minimal (control) Minimal Minimal + vitamins Hundreds of tubes of (b) complete medium are inoculated with single ascospores Complete medum Complete (control) + amino acids (e) Conidia (asexual spores) from each culture are then tested on minimal medium Minimal medium (c) No growth on minimal medium identifies nutnitional mutant Addition of arginine to minimal medium restores growth Notes: Giycine Leucine Isoleucine eujeA Methionine auues Tyrosine ueydojdku Arginine
Beadle and Tatum Experiment X rays Conidia from the cultures that fail to grow on minimal medium are then tested on a variety of supplemented media (a) Mutagenized conidia Microscopic ascospores aro dissected and translerred Crossed with wild type one by one to culture tubes Wild type of opposite mating type (d) Minimal (control) Minimal Minimal + vitamins Hundreds of tubes of (b) complete medium are inoculated with single ascospores Complete medum Complete (control) + amino acids (e) Conidia (asexual spores) from each culture are then tested on minimal medium Minimal medium (c) No growth on minimal medium identifies nutnitional mutant Addition of arginine to minimal medium restores growth Notes: Giycine Leucine Isoleucine eujeA Methionine auues Tyrosine ueydojdku Arginine
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:Beadle and Tatum Experiment
Х гаys
Conidia from the cultures that
fail to grow on minimal medium
are then tested on a variety
of supplemented media
(a)
Mutagenized
conidia
Microscopic ascospores are
dissected and translerred
Crossed with wild type one by one to culture tubes
Wild type
of opposite mating type
(d)
Hundreds of tubes of
(b) complete medium are
inoculated with single
ascospores
Minimal
(control)
Minimal
+ amino
acids
Minimal
+ vitamins
Complete
medum
Complete
(control)
(e)
Conidia (asexual spores)
from each culture are then
tested on minimal medium
Minimal
medium
(c)
No growth on minimal medium
identifies nutritional mutant
Addition of arginine to minimal medium restores growth
Notes:
Leucine
Isoleucine
eujen
Methionine
euues
Tryptopha

Transcribed Image Text:Messelsen-Stahl Experiment
Predicted results
What is seen when centrifuging
lysed bacterial samples
Conservative mechanism
Semiconservative mechanism
N14
N14
Parental strands
light DNA
(1)
synthesized in 15SN
solution
New
New
Old
(2)
After first
doubling in 14N
heavy DNA
(3)
intermediate
DNA
After second
doubling in 14N
N14 N N5
light DNA
intermediate
DNA
Figure 29
Moleculer Cell Biology. Sith Edielon
2008 W.H.Freeman and Company
(4)
Predicted
results of
centrifugation
after
replication
Notes:
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