Answered: On the graph that relates absorbance to…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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On the graph that relates absorbance to flavin concentration, draw a horizontal line that starts at the point on the y-axis that corresponds to the absorbance of your unknown sample and ends at the curve you drew through your data points . Next draw a vertical line that starts at the point where the horizontal line intersected the curve and ends at the x-axis. The point where this vertical line intersects the x-axis corresponds to the concentration of flavin in your unknown sample. What was the letter on the tube with your unknown sample? What is the concentration of the flavin in your unknown

Experiment 1
The relationship between the concentration of flavin and Its
transmission and absorbance of light.
Graph 1
100
90
80-
70
60
50
transmission
40-
30
20
10
0.02
0.04
0.06
0.08
0.1
0.12
Flavin concentration (mM)
Graph 2
2.0-
1.8-
1.6-
1.4-
1.2-
Absorbance
1.0-
0.8-
0.6-
0.4-
0.2-
0-
0.02
0.04
0.06
0.08
0.1
0.12
Flavin concentration (mM)

4,
2.
Dye
Concentration
Tube
Absorbance
Transmission
(mM)
1
0.12
2.5%0
1.598
0.06
15.8%0
801
3
0.03
39.1%
,408
63%
0.015
a00
0.0075
1१.३%
5 (blank)
0.0
100
unknown
28.8%
541

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