Which technique would be the best for analyzing lipid composition of a membrane? freeze-fracturing TLC X-ray crystallography FRAP SDS-PAGE

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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**Question:**

Which technique would be the best for analyzing lipid composition of a membrane?

- Freeze-fracturing
- TLC
- X-ray crystallography
- FRAP
- SDS-PAGE

**Points:** 1

**Explanation:**

This question asks about the most suitable technique for analyzing lipid composition in membrane studies. Each method listed has its own applications:

- **Freeze-fracturing**: Often used for studying the structural organization of cell membranes but not specifically for lipid composition.
- **TLC (Thin Layer Chromatography)**: Widely used for separating and analyzing lipids.
- **X-ray crystallography**: Primarily used for determining the 3D structure of proteins and complex molecules, but not typically for lipid analysis.
- **FRAP (Fluorescence Recovery After Photobleaching)**: Utilized to study membrane dynamics and protein mobility rather than lipid composition.
- **SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis)**: Primarily used for protein separation and analysis, not lipids.

**Correct Answer:** TLC (Thin Layer Chromatography)
Transcribed Image Text:**Question:** Which technique would be the best for analyzing lipid composition of a membrane? - Freeze-fracturing - TLC - X-ray crystallography - FRAP - SDS-PAGE **Points:** 1 **Explanation:** This question asks about the most suitable technique for analyzing lipid composition in membrane studies. Each method listed has its own applications: - **Freeze-fracturing**: Often used for studying the structural organization of cell membranes but not specifically for lipid composition. - **TLC (Thin Layer Chromatography)**: Widely used for separating and analyzing lipids. - **X-ray crystallography**: Primarily used for determining the 3D structure of proteins and complex molecules, but not typically for lipid analysis. - **FRAP (Fluorescence Recovery After Photobleaching)**: Utilized to study membrane dynamics and protein mobility rather than lipid composition. - **SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis)**: Primarily used for protein separation and analysis, not lipids. **Correct Answer:** TLC (Thin Layer Chromatography)
Expert Solution
Answer

TLC- Chemical and immunological stains are used to find the lipids on the membrane. With the help of this technique, it is feasible to identify the structures of lipids in a small number of biological samples and purify specific lipid components that were separated on an HPTLC plate.

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