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1 2 3 4 5 6 N Sample Lysate Name volume 8 Tilapia Cod Sole Perch Tilapia Cod Sole 2 μl Perch 10 με 2 μ. 10 με 2 μl 10 μl [protein] [protein] in in assay lysate 3.9 µg/ml 19.9 µg/ml 18.2 µg/ml > 20 µg/ml 9.4 µg/ml 2 μι 10 με *assay range: 2-20 µg/ml What volume of the each fish "gel-ready" sample should you load on your gel to load 5µg of protein? > 20 µg/ml <2 µg/ml 7.3 µg/ml [protein] volume in to load 5 "gel- ready" μg sample protein 7.3 μl 4.2 μl μg protein in 20 μl 19.5 μl 4.2 μl > <

1 2 3 4 5 6 N Sample Lysate Name volume 8 Tilapia Cod Sole Perch Tilapia Cod Sole 2 μl Perch 10 με 2 μ. 10 με 2 μl 10 μl [protein] [protein] in in assay lysate 3.9 µg/ml 19.9 µg/ml 18.2 µg/ml > 20 µg/ml 9.4 µg/ml 2 μι 10 με *assay range: 2-20 µg/ml What volume of the each fish "gel-ready" sample should you load on your gel to load 5µg of protein? > 20 µg/ml <2 µg/ml 7.3 µg/ml [protein] volume in to load 5 "gel- ready" μg sample protein 7.3 μl 4.2 μl μg protein in 20 μl 19.5 μl 4.2 μl > <

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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[protein] volume [protein] [protein] in to Sample Lysate in "gel- load 5 in 20 protein in Name volume assay* lysate ready" ul sample protein 3.9 2 µl Hg/ml Tilapia 19.9 10 μι Hg/ml 18.2 2 µl Hg/ml Cod >20 14 10 μι Hg/ml 9.4 15 2 µl ug/ml Sole >20 6. 10 μι ug/ml <2 17 2 µl Hg/ml Perch 7.3 8 10 μΙ Hg/ml assay range: 2-20 µg/ml What volume of the each fish "gel-ready" sample should you load on your gel to load 5ug of protein? Tilapia 7.3 ul Cod 4.2 ul Sole 19.5 ul Perch 4.2 ul > > >
Transcribed Image Text:[protein] volume [protein] [protein] in to Sample Lysate in "gel- load 5 in 20 protein in Name volume assay* lysate ready" ul sample protein 3.9 2 µl Hg/ml Tilapia 19.9 10 μι Hg/ml 18.2 2 µl Hg/ml Cod >20 14 10 μι Hg/ml 9.4 15 2 µl ug/ml Sole >20 6. 10 μι ug/ml <2 17 2 µl Hg/ml Perch 7.3 8 10 μΙ Hg/ml assay range: 2-20 µg/ml What volume of the each fish "gel-ready" sample should you load on your gel to load 5ug of protein? Tilapia 7.3 ul Cod 4.2 ul Sole 19.5 ul Perch 4.2 ul > > >
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