1. What tests should be undertaken to identify each unkown bacterium in this case study? 2. Discuss atypical results, discrepancies, or other problems that may affect the idetification3. What is the rationale behind for the choice of tests undertaken to identify the unknown bacterium? Putting It All TogetherIdentification ofUnknown Bacteriao.OOOOOCASE FILEA 5-year-old male is brought to the emergency room with anelevated temperature of 39.1°C (102.4°F), chest pain, andbreathing difficulty. Physical examination reveals crackles anddiminished lung sounds over the left lung field. His historyindicates that he has not received routine childhood vacci-nations. Initial laboratory testing shows an elevated whiteblood cell count with 89% neutrophils and elevated C-reactiveprotein. Staining of sputum shows both gram-positive and gram-negative bacteria, indicative of a mixed bacterial infection.Additional testing is undertaken to identify each bacterium.C

Disclaimer: As per Bartleby guidelines,we can answer only one question at this point of time.Additional questions that have been posted belong to clinical microbiology subject. Bacterial pneumococcal infections are very common among children . Pneumococcal vaccine in the child age group needs to be administered to prevent bacterial pneumonia kind of conditions.I.Above case is a suspected case of bacterial pneumonia.Tests that need to be prescribed for identifying bacteria in case of above mentioned symptoms is as follows: 1)Blood Culture sensitivity test- A venous blood sample is obtained to detect the presence of bacterium responsible for the infection(eg: streptococcus pneumonia).In addition, the sensitive antibiotics are also identified and thereafter notified to the physician. 2)PCR -PCR or polymerised chain reaction test is a procedure wherein the bacterial dna is used to identify the specific organism responsible for the lung infection and thereby antibiotics are prescribed for the same.