4. What is the volume of broth that can be picked up by a loopful of a sterile inoculating loop? How many bacteria would be contained in a loopful of broth from a culture at 7.3 x 105 CFU/ml?
4. What is the volume of broth that can be picked up by a loopful of a sterile inoculating loop? How many bacteria would be contained in a loopful of broth from a culture at 7.3 x 105 CFU/ml?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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4. What is the volume of broth that can be picked up by a loopful of a sterile inoculating
loop? How many bacteria would be contained in a loopful of broth from a culture at 7.3 x
10 CFU/ml?
5. What is the physical appearance (solid, liquid, semi-solid) of the following media:
broth tube, agar slant tube, agar deep tube, and agar plate. What are you testing when
you introduce an inoculum with a needle on an agar deep tube?
6. Which one is more suitable for long-term storage: agar slant tube or agar plate?
Explain.
7. There are several growth patterns on slants (e.g. filiform). Draw five different growth
patterns and do not forget to label their pattern.
8. Pigment or color (e.g. opaque, translucid, red, yellow, rose, violet, etc..) is also
another characteristic that can be used to ldentify your microorganism in a slant. Thus,
write down the name of three microorganisms (using scientific notation: Escherichia
coli) that present a pigmented pattern of growth in a slant (write down the color of each
E E kan
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