Please help! QUESTION 4Examine the results of the disk diffusion assay shown below.The bacterial strain used is most like to be resistant to which antibiotic? Consider only those identified by a letter.You do not have a ZOI table, but assume that the ZOI required to qualify a strain as resistant is the same for all antibiotics used. (In reality unliklely)O A0000ABCDOCBA

Disk diffusion assay is an assay which detects whether an antibiotic or a drug has any inhibitory…

Answered: The bacterial strain used is most like…

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Step 1- Disk diffusion assay

Disk diffusion assay is an assay which detects whether an antibiotic or a drug has any inhibitory effect on bacterial growth.
This test is conducted in laboratories, by inoculating the solidified surface of an agar plate with target bacteria and then placing antibiotic-containing paper disks to the agar. The plate is then incubated.
If an antibiotic has positive effect on stopping the bacterial growth, there will be an empty area around the disk. This area where the bacteria have not grown, if enough to be visible, is called a zone of inhibition or (ZOI).
The susceptibility of the bacterial isolate to each antibiotic can then be assessed by comparing the size of these ZOI to databases of information.
Based on the ZOI size , bacteria can be antibiotic-susceptible, moderately susceptible or resistant (if no ZOI is formed).