ple Cell Lysate Protein Concentrations

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Brad ford
Part V. Whole Cell Lysate Protein Concentrations
cell Plate Abs
HI- control
H2=0.450
SAMPLE
(Total Protein] (mg/mL)
(w/ Dilution Factor)
ТОTAL
TOTAL* Dilution
Assay
Abssos
Growth
Diet
Vol. (uL)
Vol. (uL)
Factor
YPAP
200
450
1,000
***TOTAL Dilution Factor INCLUDES pre-Dilution factor x Assay Dilution factor!
Sample Total Protein Concentration and Dilution Calculations
Please show how you calculated the total protein concentration for one of your lysates, then
how you would construct a 100 µl sample diluted to a working concentration of 1.0 mg/mL. If your
diet has a total protein concentration LOWER THAN 1.0 mg/ml, then show how you would dilute your
diet down to 0.1 mg/ml instead! As always, don't forget to MIND YOUR UNITS!!!
Transcribed Image Text:Brad ford Part V. Whole Cell Lysate Protein Concentrations cell Plate Abs HI- control H2=0.450 SAMPLE (Total Protein] (mg/mL) (w/ Dilution Factor) ТОTAL TOTAL* Dilution Assay Abssos Growth Diet Vol. (uL) Vol. (uL) Factor YPAP 200 450 1,000 ***TOTAL Dilution Factor INCLUDES pre-Dilution factor x Assay Dilution factor! Sample Total Protein Concentration and Dilution Calculations Please show how you calculated the total protein concentration for one of your lysates, then how you would construct a 100 µl sample diluted to a working concentration of 1.0 mg/mL. If your diet has a total protein concentration LOWER THAN 1.0 mg/ml, then show how you would dilute your diet down to 0.1 mg/ml instead! As always, don't forget to MIND YOUR UNITS!!!
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