Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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![Brad ford
Part V. Whole Cell Lysate Protein Concentrations
cell Plate Abs
HI- control
H2=0.450
SAMPLE
(Total Protein] (mg/mL)
(w/ Dilution Factor)
ТОTAL
TOTAL* Dilution
Assay
Abssos
Growth
Diet
Vol. (uL)
Vol. (uL)
Factor
YPAP
200
450
1,000
***TOTAL Dilution Factor INCLUDES pre-Dilution factor x Assay Dilution factor!
Sample Total Protein Concentration and Dilution Calculations
Please show how you calculated the total protein concentration for one of your lysates, then
how you would construct a 100 µl sample diluted to a working concentration of 1.0 mg/mL. If your
diet has a total protein concentration LOWER THAN 1.0 mg/ml, then show how you would dilute your
diet down to 0.1 mg/ml instead! As always, don't forget to MIND YOUR UNITS!!!](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fe78133b7-0d99-4ea8-8df6-08fe4e53086c%2Fa5ffe72f-de76-4b4e-aff4-320c4b513113%2Fykx9ak_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Brad ford
Part V. Whole Cell Lysate Protein Concentrations
cell Plate Abs
HI- control
H2=0.450
SAMPLE
(Total Protein] (mg/mL)
(w/ Dilution Factor)
ТОTAL
TOTAL* Dilution
Assay
Abssos
Growth
Diet
Vol. (uL)
Vol. (uL)
Factor
YPAP
200
450
1,000
***TOTAL Dilution Factor INCLUDES pre-Dilution factor x Assay Dilution factor!
Sample Total Protein Concentration and Dilution Calculations
Please show how you calculated the total protein concentration for one of your lysates, then
how you would construct a 100 µl sample diluted to a working concentration of 1.0 mg/mL. If your
diet has a total protein concentration LOWER THAN 1.0 mg/ml, then show how you would dilute your
diet down to 0.1 mg/ml instead! As always, don't forget to MIND YOUR UNITS!!!
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