Plot based on chart
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
Plot based on chart

Transcribed Image Text:tube # mg protein/tube protein (4 mg/ml)
1
0x4=0
0
2
0-5x4=2
0.5
3 1.0×4 = 4.0
1.0
4
15x4l0
1.5
5
2.0x4=8-0
2.0
tube # ml albumin ml globulin ml 0.85%
6
7
8
o
ml of stock human serum m1 of 0.85%
saline
2.0
1.5
1.0
0.5
0
9
0.2
0.4
o✓
o✓
0
0
1.0
2.0
saline Biuret
1.8 ✓
✓
1.6
✓
1.0
0 ✓
3.0 0.10
3.0 0.25
30
0.01
3.0
0.02
ml
Biuret
3.0
3.0
3.0
3.0
3.0
79.1%
56.9%
101-7%
104.8%
A540 %T
0
0.11
0-18
0-31
0-38
A540 %T540 mg protein mg/ml protein
in tube
in tube
540
100%
77-6%
66-1%
48.5%
41-3%

Transcribed Image Text:paper,
2. Plot your standard curve (absorbance vs. concentration in mg protein/tube) on
graph
as in Fig. 5, and illustrate on your graph how the unknown
concentrations (in mg protein/tube) are determined. Then calculate mg protein/ml
protein by dividing the mg protein/tube by the m1 of albumin or globulin you added
to each tube.
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