You perform a Bradford assay. You obtain the absorbance values listed below from the BSA samples; your protein sample yields an absorbance of 1.3; what is the protein concentration of your sample? How did you determine that?
You perform a Bradford assay. You obtain the absorbance values listed below from the BSA samples; your protein sample yields an absorbance of 1.3; what is the protein concentration of your sample? How did you determine that?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:You perform a Bradford assay. You obtain the absorbance values listed below from the BSA samples;
your protein sample yields an absorbance of 1.3; what is the protein concentration of your sample? How
did you determine that?
BSA (ug/ml)
Absorbance @ 595 nm.
25
0.15
50
0.30
75
0.45
100
0.60
150
0.90
200
1.25
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