1. Isoelectric focusing. A digested protein sample was applied to one end of a gel strip with an immobilized pH gradient. According to the experimental image from the results by gel running. Please indicated that what amino acids were compositionally enriched in separated dot 1 and 2? pH 9 Dot An electric field is applied Dot Decreasing pl pH 3 After staining, proteins are shown to be distributed along pH gradient according to their pl values.

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