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The graph given below shows the results of an experiment in which the UV absorbance (A280) of a protein solution was measured at different temperatures. One curve was obtained with the protein n ordinary buffer, pH 7. The other curve was obtained in the same buffer, and 1 M guanidinium nydrochoride (GdnHCI) was also present in the mixture. 1.0

The graph given below shows the results of an experiment in which the UV absorbance (A280) of a protein solution was measured at different temperatures. One curve was obtained with the protein n ordinary buffer, pH 7. The other curve was obtained in the same buffer, and 1 M guanidinium nydrochoride (GdnHCI) was also present in the mixture. 1.0

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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The graph given below shows the results of an experiment in which the UV absorbance (A280) of a protein solution was measured at different temperatures. One curve was obtained with the protein in ordinary buffer, pH 7. The other curve was obtained in the same buffer, and 1 M guanidinium hydrochoride (GdnHCI) was also present in the mixture. 1.0 0.8 Curve a Curve b 0.6 A280 0.4 0.2 0.0 20 40 60 80 100 temperature Which curve goes with which condition? Explain briefly how you decided.
Transcribed Image Text:The graph given below shows the results of an experiment in which the UV absorbance (A280) of a protein solution was measured at different temperatures. One curve was obtained with the protein in ordinary buffer, pH 7. The other curve was obtained in the same buffer, and 1 M guanidinium hydrochoride (GdnHCI) was also present in the mixture. 1.0 0.8 Curve a Curve b 0.6 A280 0.4 0.2 0.0 20 40 60 80 100 temperature Which curve goes with which condition? Explain briefly how you decided.
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