A mixture of species of A andB is injected into a liquid chromatographic column with length of 20.0 cm. The obtained separation results are listed as follows: Species Retention time (min) Peak Width at Base (min) Non-retained 3.0 A 5.2 0.48 5.9 0,56 From the data, the calculated resolution Rs is smaller than 1.5 for A and B when the column length is 20.0 cm, which indicates that A and B are not separate completely. What is the new column length (unit: cm) necessary to separate the A and B species with a good resolution Rs = 1.5? Please keep your answer to one decimal place.
A mixture of species of A andB is injected into a liquid chromatographic column with length of 20.0 cm. The obtained separation results are listed as follows: Species Retention time (min) Peak Width at Base (min) Non-retained 3.0 A 5.2 0.48 5.9 0,56 From the data, the calculated resolution Rs is smaller than 1.5 for A and B when the column length is 20.0 cm, which indicates that A and B are not separate completely. What is the new column length (unit: cm) necessary to separate the A and B species with a good resolution Rs = 1.5? Please keep your answer to one decimal place.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:A mixture of species of A and B is injected into a liquid chromatographic column with length of 20.0 cm. The obtained separation results are listed as follows:
Species
Retention time (min)
Peak Width at Base (min)
Non-retained
3.0
A
5.2
0.48
В
5.9
0.56
From the data, the calculated resolution Rs is smaller than 1.5 for A and B when the column length is 20.0 cm, which indicates that A and B are not separate completely. What is the
new column length (unit: cm) necessary to separate the A and B species with a good resolution Rs = 1.5? Please keep your answer to one decimal place.
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