You wish to quantify your favourite compound in an experimental solution using spectrophotometry. First, you use standard solutions of your favourite compound to collect the following standard curve data. Calculate the slope of the line of best fit to two decimal places, and enter it in the box below the table.
You wish to quantify your favourite compound in an experimental solution using spectrophotometry. First, you use standard solutions of your favourite compound to collect the following standard curve data. Calculate the slope of the line of best fit to two decimal places, and enter it in the box below the table.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
![You wish to quantify your favourite compound in an experimental solution using
spectrophotometry. First, you use standard solutions of your favourite compound to
collect the following standard curve data. Calculate the slope of the line of best fit
to two decimal places, and enter it in the box below the table.
Standard curve data
Conc (MM)
0.300
0.600
0.900
1.200
1.500
Abs
0.225
0.450
0.675
0.900
1.125
Next, you prepare an experimental sample by mixing 0.25 mL of the compound
solution with 9.75 mL of distilled water. Calculate the dilution factor and enter it as a
whole number (no decimals).
Next, you read an absorbance of 0.72 absorbance units for the diluted sample.
Calculate the concentration (mm) of the original solution of the compound. Round
this answer to one decimal place.
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Transcribed Image Text:You wish to quantify your favourite compound in an experimental solution using
spectrophotometry. First, you use standard solutions of your favourite compound to
collect the following standard curve data. Calculate the slope of the line of best fit
to two decimal places, and enter it in the box below the table.
Standard curve data
Conc (MM)
0.300
0.600
0.900
1.200
1.500
Abs
0.225
0.450
0.675
0.900
1.125
Next, you prepare an experimental sample by mixing 0.25 mL of the compound
solution with 9.75 mL of distilled water. Calculate the dilution factor and enter it as a
whole number (no decimals).
Next, you read an absorbance of 0.72 absorbance units for the diluted sample.
Calculate the concentration (mm) of the original solution of the compound. Round
this answer to one decimal place.
Previous Page
Next Page
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Page 5 of 13
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