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- What is the driving force behind paper chromatography?Define each of the following terms: A) What is resolution and how is resolution related to the wavelength of light used to illuminate the sample? B) What is the magnification of the specimen if you are using a 40x objective and a 10x eyepiece? C) How is the numerical aperture (NA) of a lens related to its ability to gather light from a specimen?What is the basic purpose of the paper chromatography?
- What are the light sources used in UV-vis spectrophotometry.How can you tell if the current or the gel electrophoresis is already running and what are the common mistakes or problems encountered in gel electrophoresis analysis?The following image is a scheme for serial dilutions prepared for spectrophotometric analysis. If the stock solution concentration is 0.05 % (v/v) can you calculate the other tube’s concentrations in % v/v? I've used this with direct dilutions, how would I use this on serial dilutions?
- What is the difference between normal and reverse phase chromatography?Describe the key components and principles of operation of a x-ray unit used for film/screen mammography.Use diagrams,where appropriate to illustrate your answerThe field of view (FOV) is the entire circular image we see when looking into the eyepiece. The diameter of the FOV gets smaller as we increase magnification. It can be measured by using a stage micrometer like a ruler, measuring from edge to edge. Notice that the stage micrometer is 1000 microns (µm) in length, and the field of view under the lowest magnification is 5000 µm. Describe how we measure it?