* You can keep extra decimal places through most of this problem, but round your final estimate to one decimal place* You wish to quantify your favourite compound in an experimental solution using spectrophotometry. First, you use standard solutions of your favourite compound to collect the following standard curve data. Calculate the slope of the line of best fit. Standard curve data
* You can keep extra decimal places through most of this problem, but round your final estimate to one decimal place* You wish to quantify your favourite compound in an experimental solution using spectrophotometry. First, you use standard solutions of your favourite compound to collect the following standard curve data. Calculate the slope of the line of best fit. Standard curve data
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:* You can keep extra decimal places through most of this problem, but round your
final estimate to one decimal place*
You wish to quantify your favourite compound in an experimental solution using
spectrophotometry. First, you use standard solutions of your favourite compound to
collect the following standard curve data. Calculate the slope of the line of best fit.
Standard curve data
Conc (mM)
0.300
0.600
0.900
1.200
1.500
Abs
0.150
0.300
0.450
0.600
0.750
Next, you prepare an experimental sample by mixing 0.50 mL of the compound
solution with 9.50 mL of distilled water, and you read an absorbance of 0.625 for this
sample. Calculate the concentration (mM) of the original solution of compound.
Round this answer to one decimal place.
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