Gel electrophoresis M FIGURE 10-14 Mixtures of different- size DNA fragments have been separated electrophoretically on an agarose gel. The samples are eight recombinant vectors treated with EcoRI. The mixtures are applied to wells near the top of the gel, and fragments move from the negative to the positive end under the influence of an electrical field to different positions dependent on size. The DNA bands have been visualized by staining with ethidium bromide and photographing under ultraviolet light. (The letter M represents lanes containing standard fragments acting as markers for estimating DNA length.) [Ingram Publishing/Thinkstock Direction of migrafion

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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In Figure 10-14, why does DNA migrate to the anode
(+ pole)?

Gel electrophoresis
M
FIGURE 10-14 Mixtures of different-
size DNA fragments have been
separated electrophoretically on an
agarose gel. The samples are eight
recombinant vectors treated with EcoRI.
The mixtures are applied to wells near
the top of the gel, and fragments move
from the negative to the positive end
under the influence of an electrical field
to different positions dependent on size.
The DNA bands have been visualized
by staining with ethidium bromide and
photographing under ultraviolet light.
(The letter M represents lanes
containing standard fragments acting
as markers for estimating DNA length.)
[Ingram Publishing/Thinkstock
Direction of migrafion
Transcribed Image Text:Gel electrophoresis M FIGURE 10-14 Mixtures of different- size DNA fragments have been separated electrophoretically on an agarose gel. The samples are eight recombinant vectors treated with EcoRI. The mixtures are applied to wells near the top of the gel, and fragments move from the negative to the positive end under the influence of an electrical field to different positions dependent on size. The DNA bands have been visualized by staining with ethidium bromide and photographing under ultraviolet light. (The letter M represents lanes containing standard fragments acting as markers for estimating DNA length.) [Ingram Publishing/Thinkstock Direction of migrafion
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