You have isolated 8 mutants in yeast that fail to grow on minimal media plates but do grow when theyare supplemented with Arginine. You know that Arginine is synthesized in a biochemical pathway withinwild-type yeast, but you do not know how many gene products it takes for the pathway. You have all of thelines as both a and a cells and mate each strain to each other in pairwise crosses and plate them on minimalmedia to see if they grow. You obtain the following results with (+) representing growth, and (-) indicatingno growth:a151a45678How many genes are represented?O 137O Cannot tell from the dataa + + + + + • + + i0 +, + + + • + +7 + + + + + , . + ++ + +m + + + + + + +2 + + + + + i +-I + + . . + + +

This is a question from classical genetics. Here one mutant strain is crossed with other mutant to…

Answered: You have isolated 8 mutants in yeast…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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An interrupted mating study is carried out on Hfr strains 1, 2, and 3. After conjugation is established, a small sample of the mixture is collected every minute for 20 minutes to determine the distance between the genes on the chromosome. Times of entry for each of the three Hfr strains studied in the interrupted mating are provided in parenthesis. The total duration of conjugation (in minutes) is given for each transferred gene. Hfr Strain Genes Transferred Hfr 1 oriT(0) met(2) ala(8) lac(13) gal(17) Hfr 2 oriT(0) met(2) leu(7) thr(10) azi(17) Hfr 3 oriT (0) gal(3) pro(8) trp(14) azi(19) Hfr 4 oriT leu met ala lac Hfr 5 oriT trp azi thr leu a. Use the data to construct a circular map of the bacterium.
Based on the attached image, if we are using the Holliday junction model of recombination, where exactly would be the positions where DNA is cut? Would it be to the right because of branch migration?
Please find the attachment
An Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data points shown in the following graph,the conjugation was interrupted at different time points, and thepercentage of recombinants for each gene was determined bystreaking on a medium that lacked either leucine or thiamine.What information do you know based on the question and your understanding of the topic?
Two strains of S. cerevisae (yeast) are crossed. One has the genotype A B and the other a b. Which statement correctly describes the tetrads that can be produced by one reciprocal exchange between the A and B genes without gene conversion in the dihybrid? A) Recombination occurs at the four-strand stage to produce a tetrad with A B, A B, a B, and a b chromosomes. B) Recombination occurs at the four-strand stage to produce a tetrad with A B, A b, a B, and a b chromosomes. C) Recombination occurs at the two-strand stage to produce a tetrad with A B, A b, a B, and a b chromosomes. D) Recombination occurs at the two-strand stage to produce a tetrad with A b, A b, a B, and a B chromosomes.
What information would you need to tell whether the leading and lagging strands in yeast are replicated with equal fidelity?
In Figure 6-13, explain at the protein level why this heterokaryon can grow on minimal medium
From an Escherichia coli strain, five Hfr strains were isolated. The location and orientation of the transfer origin of each Hfr strain is shown in Figure 1. You want to use these five strains to map the locus responsible for thiamine synthesis, called thi. Each Hfr strain is sensitive to rifampicin (Rifs) and thi*. Conjugation experiments are performed between each of the Hfr strains and an F strain Rif Thi™. 0 T leu 10 20 T nadD pyrC trp 40 T his 60 70 2) The results are shown in the following table: Donor strain Hfr1 Hfr2 Hfr3 Hfr4 Hfr5 cysG 80 90 1) What is the selection medium used in these conjugation experiments metA Colonies Thi+ 1000 0 400 0 25 100 Hfr1 Hfr2 Figure 1: Chromosome map of Escherichia coli. Five Hfr strains (Hfr1 to Hfr5) were isolated and the location and orientation of the origin of transfer is shown by the arrows in each Hfr strain. Distances in minutes are shown. Leu: leucine biosynthesis; nadD: NAD biosynthesis; pyrC: pyrimidine biosynthesis; trp: tryptophan…
You are a developmental geneticist studying flowering time variation in Arabidopsis. You perform a mutagenesis screen to identify mutants in the photoperiod pathway. You conduct the screen and find two different plants that show the same mutant phenotype. You then use a complementation test. What is the predicted outcome of this test if both phenotypes are caused by mutations in separate genes? recover the wild type phenotype overexpress the gene O recover the mutant phenotype
An Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data points shown in the following graph,the conjugation was interrupted at different time points, and thepercentage of recombinants for each gene was determined bystreaking on a medium that lacked either leucine or thiamine.Make a calculation.

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