Linkage maps in an Hfr bacterial strain are calculated in units of minutes (the number of minutes between genes indicates the length of time that it takes for the second gene to follow the first in conjugation). In making such maps, microbial geneticists assume that the bacterial chromosome is transferred from Hfr to F − at a constant rate. Thus, two genes separated by 10 minutes near the origin end are assumed to be the same physical distance apart as two genes separated by 10 minutes near the F − attachment end. Suggest a critical experiment to test the validity of this assumption.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Linkage maps in an Hfr bacterial strain are calculated in
units of minutes (the number of minutes between genes
indicates the length of time that it takes for the second
gene to follow the first in conjugation). In making such
maps, microbial geneticists assume that the bacterial
chromosome is transferred from Hfr to F
− at a constant
rate. Thus, two genes separated by 10 minutes near the
origin end are assumed to be the same physical distance
apart as two genes separated by 10 minutes near the F
−
attachment end. Suggest a critical experiment to test the
validity of this assumption.
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