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FIGURE 5-11 After conjugation, crossovers are needed to integrate genes from the donor fragment into the recipient's chromosome and, hence, Crossovers integrate parts of the transferred donor fragment Hfr F- become a stable part of its genome. LaunchPad ANIMATED ART: Bacterial conjugation and recombination Transfer of single-stranded DNA copy b- a Exconjugant Transferred fragment converted into double helix Exogenote Endogenote- Recombinant -Lost Double crossover inserts donor DNA Putting all these observations together, Wollman and Jacob deduced that, in the conjugating Hfr, single-stranded DNA transfer begins from a fixed point on the donor chromosome, termed the origin (O), and continues in a linear fashion. The point O is now known to be the site at which the F plasmid is inserted. The farther a gene is from 0, the later it is transferred to the F~. The transfer process will generally stop before the farthermost genes are transferred, and, as a result, these genes are included in fewer exconjugants. Note that a type of chromosome map can be produced in units of minutes, based on time of entry of marked genes. In the example in Figure 5-12, the map would be:

FIGURE 5-11 After conjugation, crossovers are needed to integrate genes from the donor fragment into the recipient's chromosome and, hence, Crossovers integrate parts of the transferred donor fragment Hfr F- become a stable part of its genome. LaunchPad ANIMATED ART: Bacterial conjugation and recombination Transfer of single-stranded DNA copy b- a Exconjugant Transferred fragment converted into double helix Exogenote Endogenote- Recombinant -Lost Double crossover inserts donor DNA Putting all these observations together, Wollman and Jacob deduced that, in the conjugating Hfr, single-stranded DNA transfer begins from a fixed point on the donor chromosome, termed the origin (O), and continues in a linear fashion. The point O is now known to be the site at which the F plasmid is inserted. The farther a gene is from 0, the later it is transferred to the F~. The transfer process will generally stop before the farthermost genes are transferred, and, as a result, these genes are included in fewer exconjugants. Note that a type of chromosome map can be produced in units of minutes, based on time of entry of marked genes. In the example in Figure 5-12, the map would be:

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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In Figure 5-11, which donor alleles become part of the
recombinant genome produced?

FIGURE 5-11 After conjugation, crossovers are needed to integrate genes from the donor fragment into the recipient's chromosome and, hence, Crossovers integrate parts of the transferred donor fragment Hfr F- become a stable part of its genome. LaunchPad ANIMATED ART: Bacterial conjugation and recombination Transfer of single-stranded DNA copy b- a Exconjugant Transferred fragment converted into double helix Exogenote Endogenote- Recombinant -Lost Double crossover inserts donor DNA Putting all these observations together, Wollman and Jacob deduced that, in the conjugating Hfr, single-stranded DNA transfer begins from a fixed point on the donor chromosome, termed the origin (O), and continues in a linear fashion. The point O is now known to be the site at which the F plasmid is inserted. The farther a gene is from 0, the later it is transferred to the F~. The transfer process will generally stop before the farthermost genes are transferred, and, as a result, these genes are included in fewer exconjugants. Note that a type of chromosome map can be produced in units of minutes, based on time of entry of marked genes. In the example in Figure 5-12, the map would be:
Transcribed Image Text:FIGURE 5-11 After conjugation, crossovers are needed to integrate genes from the donor fragment into the recipient's chromosome and, hence, Crossovers integrate parts of the transferred donor fragment Hfr F- become a stable part of its genome. LaunchPad ANIMATED ART: Bacterial conjugation and recombination Transfer of single-stranded DNA copy b- a Exconjugant Transferred fragment converted into double helix Exogenote Endogenote- Recombinant -Lost Double crossover inserts donor DNA Putting all these observations together, Wollman and Jacob deduced that, in the conjugating Hfr, single-stranded DNA transfer begins from a fixed point on the donor chromosome, termed the origin (O), and continues in a linear fashion. The point O is now known to be the site at which the F plasmid is inserted. The farther a gene is from 0, the later it is transferred to the F~. The transfer process will generally stop before the farthermost genes are transferred, and, as a result, these genes are included in fewer exconjugants. Note that a type of chromosome map can be produced in units of minutes, based on time of entry of marked genes. In the example in Figure 5-12, the map would be:
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