Bacteriophage λ, after infecting a cell, can integrate into the chromosome of the cell if the repressor protein, cI, binds to and shuts down phage transcription immediately. (A strain containing a bacteriophage DNA integrated into the chromosome is called a lysogen.) The alternative fate is the production of many more viruses and lysis of the cell. In a mating, a donor strain that is a lysogen was crossed with a lysogenic recipient cell, and no phages were produced. However, when the lysogen donor strain transferred its DNA to a nonlysogenic recipient cell, the recipient cell burst, releasing a new generation of phages. b. Explain how this phenomenon relates to the PaJaMo experiment in Fig. 16.6
Bacteriophage λ, after infecting a cell, can integrate into the chromosome of the cell if the repressor protein, cI, binds to and shuts down phage transcription immediately. (A strain containing a bacteriophage DNA integrated into the chromosome is called a lysogen.) The alternative fate is the production of many more viruses and lysis of the cell. In a mating, a donor strain that is a lysogen was crossed with a lysogenic recipient cell, and no phages were produced. However, when the lysogen donor strain transferred its DNA to a nonlysogenic recipient cell, the recipient cell burst, releasing a new generation of phages. b. Explain how this phenomenon relates to the PaJaMo experiment in Fig. 16.6
Human Anatomy & Physiology (11th Edition)
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Bacteriophage λ, after infecting a cell, can integrate
into the chromosome of the cell if the repressor protein,
cI, binds to and shuts down phage transcription
immediately. (A strain containing a bacteriophage
DNA integrated into the chromosome is called a lysogen.)
The alternative fate is the production of many
more viruses and lysis of the cell. In a mating, a donor
strain that is a lysogen was crossed with a lysogenic
recipient cell, and no phages were produced.
However, when the lysogen donor strain transferred
its DNA to a nonlysogenic recipient cell, the recipient
cell burst, releasing a new generation of phages.
b. Explain how this phenomenon relates to the
PaJaMo experiment in Fig. 16.6

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16.2 Regulation of Transcription Initiation via DNA-Binding Proteins
553
other molecule. Jacob and Monod thus required a major
leap of imagination in order to propose their theory.
We now know that a key concept of the theory-that
proteins bind to DNA to regulate gene expression-holds
true for the positive as well as the negative regulation of the
lac operon. The binding of proteins to DNA is also central
to the control of many prokaryotic genes outside the lac
operon, including the inducible regulation of other cata-
bolic genes and the repressible regulation of anabolic
genes, and to eukaryotic genes as well.
Figure 16.6 The PaJaMo experiment. DNA carrying lacl*
and lacz* genes is introduced (by conjugation) into a lacl lacZ
cell. In the recipient, B-galactosidase is synthesized from the
introduced lacZ* gene initially, but as repressor (made from the
introduced laci“) accumulates, the synthesis of B-galactosidase
stops. If inducer is added (dotted line), the synthesis of
B-galactosidase resumes.
+ inducer
Genetic Analysis Led Jacob and Monod
to the Operon Hypothesis
No inducer
lacl* lacz+
On the way to developing the operon theory of gene regula-
tion, Monod and his collaborators isolated many different
mutations that either prevented the cells from utilizing lac-
tose or that allowed the cells to synthesize the enzymes
genes introduced
needed to break down lactose all the time, whether lactose
was present in the environment or not.
2
3
4
6
7
8.
Time (hours)
Complementation and mapping analyses
of Lac¯ mutants
Lac mutants are bacterial cells unable to utilize lactose.
The historic PaJaMo experiment–named after Arthur
MAY
w
amount of B-galactosidase
synthesis in lacl- lacZ¯ mutant
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