You've crossed two strains: (Hfr) pro+ lac+ arg+ with (F-) pro- lac- arg- .Interrupted mating studies indicated that arg+ enters the cell last, so you want toselect for arg+ recombinants in order to map the genes by recombination. Whichtype of plates will you use for your initial selection?Containing proline, lactose, and arginine.Containing proline, glucose, and arginine.Containing arginine and glucose.Containing lactose and proline.Containing glucose and proline.Minimal media with nothing added.

Introduction: When F factor integates into the main chromosome the cell known as Hfr starin. It…

Answered: You've crossed two strains: (Hfr) pro+…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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The pathway for arginine biosynthesis in Neurospora crassa involves several enzymes that produce a series of intermediates as shown. O O O O ornithine citrulline ARG-E arginosuccinate arginine N-acetylornithine arginine You did a cross between ARG-E ARG-H* and ARG-E* ARG-H¯¯ Neurospora strains and identified an Arg- strain from an NPD tetrad. (Assume that Neurospora forms tetrads in the same way yeast do.) Which compound would rescue growth of this Arg- spore? N-aceltylornithine ARG-F ornithine citrulline ARG-G ARG-H → argininosuccinate
1) Using the diagram below, sketch in the pattern of bands you would expect to see after digesting the DNA of the TT, Tt and tt genotypes of the TAS2R38 gene. Use the Base Pair Standards on the left of the diagram as a reference in drawing the positions of the bands. Base Pair TASTER GENO TYPE Standards (Base Pairs) TT Tt tt 500 400 300 200 100 50
Based on the attached image, if we are using the Holliday junction model of recombination, where exactly would be the positions where DNA is cut? Would it be to the right because of branch migration?
The genomes of two E. coli strains are compared in Figure14-19. Would you expect any third strain to contain moreof the blue, tan, or red regions shown in Figure 14-19?Explain.
An Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data points shown in the following graph,the conjugation was interrupted at different time points, and thepercentage of recombinants for each gene was determined bystreaking on a medium that lacked either leucine or thiamine.What information do you know based on the question and your understanding of the topic?
The pathway for arginine biosynthesis in Neurosporacrassa involves several enzymes that produce a seriesof intermediates.ARG-E ARG-F ARG-G ARG-HN-acetylornithine → ornithine → citrulline → argininosuccinate → argininea. If you did a cross between ARG-E− and ARG-H−Neurospora strains, what would be the distributionof Arg+ and Arg− spores within parental ditype andnonparental ditype asci? Give the spore types inthe order in which they would appear in the ascus.b. For each of the spores in your answer to part (a),what nutrients could you supply in the media to getspore growth?
SOD1 vs RM95 – 20% recombinant TGLA57 vs UMPS – 21% recombinant UMPS vs CRYG8- 17% Recombinant RM95 vs UMPS 50% - recombinant TGLA vs CRYG8 – 38% recombinant SOD1 vs TGLA57 – 49% recombinant RM95 vs TGLA 57 – 29% recombinant SOD1 vs UMPS – 50% recombinant what is the order of the genes on the chromosome and what is there distance in cM
A pair of paralogous repeats, A and B, have 96% sequence similarity and therefore can promote non-allelic homologous recombination (NAHR). They exist in four possible arrangements in a genome, illustrated below as arrangements 1 – 4. What is the result of NAHR between repeats A and B in arrangement 1? A.Translocation between chromosomes 1 and 2 resulting in monocentric chromosomes B.Deletion or duplication of the region between A and B C.Translocation between chromosomes 1 and 2 resulting in acentric and dicentric chromosomes D.Inversion of the region between A and B
An Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data points shown in the following graph,the conjugation was interrupted at different time points, and thepercentage of recombinants for each gene was determined bystreaking on a medium that lacked either leucine or thiamine.Make a calculation.

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