The pathway for arginine biosynthesis in Neurosporacrassa involves several enzymes that produce a seriesof intermediates.ARG-E ARG-F ARG-G ARG-HN-acetylornithine → ornithine → citrulline → argininosuccinate → argininea. If you did a cross between ARG-E− and ARG-H−Neurospora strains, what would be the distributionof Arg+ and Arg− spores within parental ditype andnonparental ditype asci? Give the spore types inthe order in which they would appear in the ascus.b. For each of the spores in your answer to part (a),what nutrients could you supply in the media to getspore growth?
Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
The pathway for arginine biosynthesis in Neurospora
crassa involves several enzymes that produce a series
of intermediates.
ARG-E ARG-F ARG-G ARG-H
N-acetylornithine → ornithine → citrulline → argininosuccinate → arginine
a. If you did a cross between ARG-E− and ARG-H−
Neurospora strains, what would be the distribution
of Arg+ and Arg− spores within parental ditype and
nonparental ditype asci? Give the spore types in
the order in which they would appear in the ascus.
b. For each of the spores in your answer to part (a),
what nutrients could you supply in the media to get
spore growth?
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