His-64 is an amino acid in myoglobin's herne-binding pocket. It is called the "distal histidine." A scientigt conducted an experiment wherein she replaced His-64 with alanine. This mutation was designated H84AA. She also created two other mutants His64AP and His64AS. She then measured the oxygen-binding ability of each of these Mb variants. Her results are given below: (a) ) What is the role of the distal Histidine in oxygen binding? ( b) ) On the graph above, observe the 4 Oxygen binding curves 1, 2, 3 and 4. Curve 1 represents normal (WT) Mb. i. Variant H644A is represented by curve #: il. Variant H64AP is represented by curve # ill. Variant H64AS is represented by curve #: (c) In 3-4 sentences, explain your answers to Part (b)
His-64 is an amino acid in myoglobin's herne-binding pocket. It is called the "distal histidine." A scientigt conducted an experiment wherein she replaced His-64 with alanine. This mutation was designated H84AA. She also created two other mutants His64AP and His64AS. She then measured the oxygen-binding ability of each of these Mb variants. Her results are given below: (a) ) What is the role of the distal Histidine in oxygen binding? ( b) ) On the graph above, observe the 4 Oxygen binding curves 1, 2, 3 and 4. Curve 1 represents normal (WT) Mb. i. Variant H644A is represented by curve #: il. Variant H64AP is represented by curve # ill. Variant H64AS is represented by curve #: (c) In 3-4 sentences, explain your answers to Part (b)
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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His-64 is an amino acid in myoglobin's herne-binding pocket. It is called the "distal histidine." A scientigt conducted an experiment wherein she replaced His-64 with alanine. This mutation was designated H84AA. She also created two other mutants His64AP and His64AS. She then measured the oxygen-binding ability of each of these Mb variants. Her results are given below:
(a) ) What is the role of the distal Histidine in oxygen binding? (
b) ) On the graph above, observe the 4 Oxygen binding curves 1, 2, 3 and 4. Curve 1 represents normal (WT) Mb.
i. Variant H644A is represented by curve #:
il. Variant H64AP is represented by curve #
ill. Variant H64AS is represented by curve #:
(c) In 3-4 sentences, explain your answers to Part (b)
Transcribed Image Text:Her results are given below:
2
3
4
1
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