In E. coli, the gene bioD+ encodes an enzyme involved in biotin synthesis, and galK+ encodes an enzyme involved in galactose utilization. An E. coli strain that contained wild-type versions of both genes was infected with P1 phage, and then a P1 lysate was obtained. This lysate was used to
transduce (infect) a strain that was bioD− and galK−. The cells
were plated on a medium containing galactose as the sole carbon
source for growth to select for transduction of the galK+ gene.
This medium also was supplemented with biotin. The resulting
colonies were then restreaked on a medium that lacked biotin to
see if the bioD+ gene had been cotransduced. The following results
were obtained:
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