Suppose you cultured yeast, that you had transformed with pMZ379 plus the AVT5 sgRNA, on a plate of YES agar plus salt ions. You then cultured the same yeast on a plate containing only YES agar. Your results showed no growth on either plate. Which of the following is the most reasonable interpretation of your results? O A) The yeast were not transformed with pMZ379. B) The Cas9 gene was mutated, and was not able to cut the DNA and silence the yeast gene. C) The sgRNA gene was mutated in the plasmid, so that it did not lead the Cas9 to the correct yeast gene. D) The yeast were not viable or the conditions of growth in the experiment (temperature, humidity, etc.) were not sufficient to promote growth of the yeast. lecture 21.m4a Apr 20, 2022 19r
Molecular Techniques
Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology are employed to investigate the molecular basis for biological activity. These techniques are used to analyze cellular properties, structures, and chemical reactions, with a focus on how certain molecules regulate cellular reactions and growth.
DNA Fingerprinting and Gel Electrophoresis
The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological samples such as blood, hair, saliva, semen can be used for DNA fingerprinting. DNA fingerprinting is also known as DNA profiling or molecular fingerprinting.
Molecular Markers
A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the first marker. In two categories, molecular markers are classified, classical marker and a DNA marker. A molecular marker is also known as a genetic marker.
DNA Sequencing
The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under the genomic field. The primary objective of the Human Genome Project was to determine the nucleotide sequence of the entire human nuclear genome. DNA sequencing selectively eliminates the introns leading to only exome sequencing that allows proteins coding.
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