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Resistance Gene." a) Using the diagram provided, describe where the forward and reverse primers would bind for amplification.

Resistance Gene." a) Using the diagram provided, describe where the forward and reverse primers would bind for amplification.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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1. You are part of a research group that wants to create a strain of humans resistant to COVID-19 by inserting the "Corona Virus Resistance Gene" into the next generation of humans. In order to achieve this, you must design primers that would amplify the "Corona Virus Resistance Gene." a) Using the diagram provided, describe where the forward and reverse primers would bind for amplification. b) Would this construct use the same primers as Dr. Devlin used in his Growth Hormone construct? Explain. Gene Gene Gene Terminator Promoter 2. a) If you started with 5 DNA strands and the above diagram repeated for 9 cycles, how th many DNA strands would you have after the 9" cycle? b) What would happen if you forgot to add dNTPs to the PCR reactions? Would any DNA amplification occur? 3. A linear DNA molecule is digested by the LoIRI restriction enzyme that cuts DNA at four different positions. After completing the restriction digest, you use gel electrophoresis to visualize the DNA fragments. Answer the following questions. a) How many bands would you observe after looking at your gel image? b) During gel electrophoresis, what must you load onto your gel so that you can determine the band sizes of each solution after running the gel?
Transcribed Image Text:1. You are part of a research group that wants to create a strain of humans resistant to COVID-19 by inserting the "Corona Virus Resistance Gene" into the next generation of humans. In order to achieve this, you must design primers that would amplify the "Corona Virus Resistance Gene." a) Using the diagram provided, describe where the forward and reverse primers would bind for amplification. b) Would this construct use the same primers as Dr. Devlin used in his Growth Hormone construct? Explain. Gene Gene Gene Terminator Promoter 2. a) If you started with 5 DNA strands and the above diagram repeated for 9 cycles, how th many DNA strands would you have after the 9" cycle? b) What would happen if you forgot to add dNTPs to the PCR reactions? Would any DNA amplification occur? 3. A linear DNA molecule is digested by the LoIRI restriction enzyme that cuts DNA at four different positions. After completing the restriction digest, you use gel electrophoresis to visualize the DNA fragments. Answer the following questions. a) How many bands would you observe after looking at your gel image? b) During gel electrophoresis, what must you load onto your gel so that you can determine the band sizes of each solution after running the gel?
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