protein. You create a mouse line with Cas9 under control of a brain-specific enhancer, while the shortguide RNA complementary to the first exon of Gene Y is expressed in all tissues. You subsequentlysequence Gene Y in both brain and liver tissue. What would expect in each tissue? You can assumethat the CRISPRICas9 system will impact both copies of Gene Y in cells, and that the first exon ofGene Y is necessary for Gene Ys function.a. Liver: Functional Gene Y; Brain: Functional Gene Yb. Liver: Nonfunctional Gene Y; Brain: Funtional Gene Yc. Liver: Functional Gene Y; Brain: Nonfunctional Gene Yd. Liver: Nonfunctional Gene Y; Brain: Nonfunctional Gene Y

CRISPR technology is a simple yet powerful tool for editing genomes. It allows researchers to easily…

Answered: protein. You create a mouse line with…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Similar questions

Match the term with its description. cAMP Allows lactose to enter the cell and product of the lacY gene A regulatory sequence between the promoter and the structural genes of the lac operon When glucose levels are high, intracellular levels of this molecule are low Binds to and inhibits the function of the lac repressor when lactose is present
Great! Now you have the gene sequence. Now you would like to clone it into an expression vector to grow up in a bacterial system. Because you're going to use bacteria to generate protein from a eukaryote, the mammoth, you need to get rid of introns from your sequence. How do you do that? O Bioinformatically, I look for splice-site sequences and branch-point adenines and predict intron-exon boundaries O l use a comparative genomic approach and use sequence homology with the genome of a closely related species O luse a comparative genomic approach and use sequence homology with the genome of a distantly related species O Both A and B O Both B and C
. You obtain the DNA sequence of a mutant of a 2-kb genein which you are interested and it shows base differencesat three positions, all in different codons. One is a silentchange, but the other two are missense changes (they encode new amino acids). How would you demonstratethat these changes are real mutations and not sequencing errors? (Assume that sequencing is about 99.9 percent accurate.)
please help. do 1st problem
73Dicer catalyzes Sequence-dependent single-stranded RNA cleavage. Yesorno 74Amino acid-coding regions within eukaryotic genes may be interrupted by ___________ regions. A.noncodingb.long terminal repeatc.split geneD.enhancer 75Which of the following cleaves the N-terminal methionine from polypeptides?A.Methionine isomeraseb.Methionine aminopeptidasec.Methionine carboxylaseD.Methionine transferase
Gene Regulation and Breast Cancer Researchers have found that breast cancer cells commonly express a transcription factor known as Ets1 at high levels. Many types of breast cancer are also sensitive to the presence of the hormone estrogen. In estrogen-sensitive breast cancers, tumors grow much more quickly when high levels of estrogen are present. A biologist decides to design an experiment to determine if there was any cooperation between estrogen and Ets1in causing tumor growth. The biologist developed two types of breast cancer cells: one that expressed Ets1 at very high levels, designated as Ets+, and one that was unable to express Ets1, designated as Ets–. The biologist used four test groups in an experiment: 1. Ets1+ cells with added estrogen 2. Ets1- cells with added estrogen 3. Ets1+ cells without estrogen 4. Ets1- cells without estrogen The growth rate of each group of cells was monitored and recorded. Use the passage to answer the question. After five days of cell…
siRNA and CRISPR/Cas9 each interfere with gene expression by a sequence-directed mechanism. A. Identify the target macromolecule (DNA, RNA or Protein) of a specific siRNA/RISC andCRISPR/Cas9 complexsiRNA:CRISPR/Cas9: B. If a scientist would like to permanently turn off expression of a particular human gene inthe liver for the lifetime of an individual, which of these techniques would bepreferable? Briefly explain why
A. Based on this data, draw a conclusion about the promoter elements that Caudal specifically upregulates. B. Dr. Juven-Gershon also tested Caudal activation of core promoter sequences from twoother genes. Similar to the first experiment, one was DPE-dependent (from the E74Bgene), and the other was TATA-dependent (from the Adh gene). However, these two corepromoters lacked the BREU motif. The results from these additional core promoters areshown below. Based on these results and those shown above, propose a hypothesis thatexplains the differences in Caudal-mediated upregulation among all the different corepromoters Dr. Juven-Gershon tested. C. To test your hypothesis from Question 3B, you plan perform the same method of genereporter assays that Dr. Juven-Gershon has done. However, you will need to do somemutations to alter the core promoters. Make diagrams or clearly describe all the variouscore promoters mutants you wish to test. These include your controls, as well as any…
Methylation state of cytosines at the QRT gene promoter is tested in one pair of identical twins, Ana and Becca, at 1 year of age, again when they are 25 years old. At 1 year old, the twins' DNA is the same, and this part of the genome is not methylated. At age 25, Ana's DNA remains unmethylated, but Becca's cells show methylation at this site in skin cells. a. What most likely caused this change in Becca? i. A mutation event ii. An environmental stimulus b. When the twins were 1 year old, QRC mRNA was expressed at similar levels in both Ana and Becca. At age 25, which twin likely expresses more of the QRC mRNA? i. Ana, whose QRC promoter DNA is unmethylated ii. Becca, whose QRC promoter DNA is methylated iii. Both are the same C. At age 25, which twin has more tightly packed chromatin in the region of the QRC gene? Explain briefly. i. Ana, whose QRC promoter DNA is unmethylated ii. Becca, whose QRC promoter DNA is methylated iii. Both are the same d. Some of Becca's skin cells, in…
Examine the network motifs in Figure Q8–5.Decide which ones are negative feedback loops and whichare positive. Explain your reasoning.
1. What features of a protein-coding gene might be found in a non-coding gene? 2. What features are unnecessary in a non-coding gene?
Molecular biology, please explain in details

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