protein. You create a mouse line with Cas9 under control of a brain-specific enhancer, while the shortguide RNA complementary to the first exon of Gene Y is expressed in all tissues. You subsequentlysequence Gene Y in both brain and liver tissue. What would expect in each tissue? You can assumethat the CRISPRICas9 system will impact both copies of Gene Y in cells, and that the first exon ofGene Y is necessary for Gene Ys function.a. Liver: Functional Gene Y; Brain: Functional Gene Yb. Liver: Nonfunctional Gene Y; Brain: Funtional Gene Yc. Liver: Functional Gene Y; Brain: Nonfunctional Gene Yd. Liver: Nonfunctional Gene Y; Brain: Nonfunctional Gene Y

CRISPR technology is a simple yet powerful tool for editing genomes. It allows researchers to easily…

Answered: protein. You create a mouse line with…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

See similar textbooks

Similar questions

a. Why is it impossible to induce nonsense mutations(represented at the mRNA level by the triplets UAG,UAA, and UGA) by treating wild-type strains with mutagens that cause only A• G → T• C transitions in DNA?b. Hydroxylamine (HA) causes only G• C → A• T transitions in DNA. Will HA produce nonsense mutations inwild-type strains?c. Will HA treatment revert nonsense mutations?
Transcriptional regulation You are interested in studying the transcriptional regulation of Glp1 promoter. This gene contains a binding site for two proteins A and B. Proteins A and B cannot bind to the DNA at the same time due to steric interference caused by a slight overlap in their binding sites. The binding sites of protein A and protein B can be seen in the figure below. Protein A binds to Site C and Protein B binds to site D. To assess whether either A or B have an influence on Glp1 expression, you create mutations in the DNA that selectively remove the non-overlapping sequences of binding site C or binding site D. You then examine Glp1 mRNA production within adult liver cells. You receive the following data. Experiment number Binding site C Binding site D Glp1 mRNA levels 1 + + high 2 + - high 3 - + none 4 - - none += binding site present, -= binding site absent What does this data tell us about which protein is…
amount of protein / amount of mRNA 12 mm / ↑ A B time (minutes) 20 Bacteria were grown in culture. At various time points, substances were added or removed from the culture medium. To assay the effects of the substances on operon gene expression, samples of bacteria were collected at one-minute intervals and levels of specific proteins and mRNAs were determined. If the operon under investigation is a repressible operon, which arrow indicates the addition of a molecule that binds the repressor protein?
. All mutations that abolish function of the Rho termination protein in E. coli are conditional mutations;no cells with null mutations of the Rho-encoding genehave ever been isolated. What does this tell you aboutthe rho gene and its product?
Like the lac operon, the hexose operon is controlled by a separate regulatory protein under the control of its own promoter (see the schematic of the operon below). The hexose regulatory protein is sensitive to fatty acyl CoA levels. When all hexose fuel sources are depleted, the bacteria switch to lipid metabolism and fatty acyl CoA levels increase. This turns expression of the hexose operon off. +1 +1 Regulatory Gene P regulator Hexose Operon Genes operon regulator promoter operon promoter e. The regulatory protein that controls expression of the hexose operons is a transcriptional ACTIVTOR or REPRESSOR (circle one).
A. Based on this data, draw a conclusion about the promoter elements that Caudal specifically upregulates. B. Dr. Juven-Gershon also tested Caudal activation of core promoter sequences from twoother genes. Similar to the first experiment, one was DPE-dependent (from the E74Bgene), and the other was TATA-dependent (from the Adh gene). However, these two corepromoters lacked the BREU motif. The results from these additional core promoters areshown below. Based on these results and those shown above, propose a hypothesis thatexplains the differences in Caudal-mediated upregulation among all the different corepromoters Dr. Juven-Gershon tested. C. To test your hypothesis from Question 3B, you plan perform the same method of genereporter assays that Dr. Juven-Gershon has done. However, you will need to do somemutations to alter the core promoters. Make diagrams or clearly describe all the variouscore promoters mutants you wish to test. These include your controls, as well as any…
Methylation state of cytosines at the QRT gene promoter is tested in one pair of identical twins, Ana and Becca, at 1 year of age, again when they are 25 years old. At 1 year old, the twins' DNA is the same, and this part of the genome is not methylated. At age 25, Ana's DNA remains unmethylated, but Becca's cells show methylation at this site in skin cells. a. What most likely caused this change in Becca? i. A mutation event ii. An environmental stimulus b. When the twins were 1 year old, QRC mRNA was expressed at similar levels in both Ana and Becca. At age 25, which twin likely expresses more of the QRC mRNA? i. Ana, whose QRC promoter DNA is unmethylated ii. Becca, whose QRC promoter DNA is methylated iii. Both are the same C. At age 25, which twin has more tightly packed chromatin in the region of the QRC gene? Explain briefly. i. Ana, whose QRC promoter DNA is unmethylated ii. Becca, whose QRC promoter DNA is methylated iii. Both are the same d. Some of Becca's skin cells, in…
All about splicing A. snRNPs interactions will bring the 5' and 3' splice sites together in the pre-mRNA B. lariat formation is necessary to bring the branch site with the 5' splice site of the intron C. an unstable 5'P and 2'OH phosphodiester bond helps form the lariat D. exon 5' splice site consensus sequence GU and its 3' splice site AG are recognized by snRNPs E. various types of snRNPs and the pre-RNA come together to form the spliceosome B, C, D, E only A, B, C, E only A, B, C, D, E A, B, C only
E17. Gene mutagenesis is also used to explore the structure and function of proteins. For example, changes can be made to the coding sequence of a gene to determine how alterations in the amino acid sequence affect the function of a protein. Let's suppose that you are interested in the functional importance of a particular glutamic acid (an amino acid) within a protein you are studying. By site-directed mutagenesis, you make mutant proteins in which this glutamic acid codon has been changed to other codons. You then test the encoded mutant proteins for functionality. The results are as follows: Functionality (%) Normal protein 100 Mutant proteins containing Тугosine Phenylalanine 3 Aspartic acid 94 Glycine From these results, what would you conclude about the functional significance of this glutamic acid within the protein?
1. enzymes that catalyze histone acetlation are closesly associated with transription factors, which are proteins that promote transcription. why is this a good biochemical strategy? 2. sp1 is a sqeuence specific human DNA binding protein that binds to a region on the DNA called the GC box, a promoter element with the sequence GGGCGG. Binding of Sp1 to the GC box enhances RNA polymerase 2 activity 50- to a 100 fold. How would you use affinity chromatography to purify SP1?
Like the lac operon, the hexose operon is controlled by a separate regulatory protein under the control of its own promoter (see the schematic of the operon below). The hexose regulatory protein is sensitive to fatty acyl CoA levels. When all hexose fuel sources are depleted, the bacteria switch to lipid metabolism and fatty acyl CoA levels increase. This turns expression of the hexose operon off. +1 +1 Regulatory Gene Pregulator P operon Hexose Operon Genes regulator promoter operon promoter
I am more confused. how about we start from begining, you post answers on here, and then we go from there? 1. Identify the open reading frame in the following DNA sequence, the protein that this gene encodes for, its function, and the source. 2. "Look carefully at the DNA sequence and identify the start site for transcription" 3. Click on the DNA sequence from the start site of transcription, select all of the sequence, and copy the sequence. Go to the National Center for Biotechnology Information (NCBI) website http://www.ncbi.nlm.nih.gov/. Click on BLAST on the right-hand side under “Popular Resources.” BLAST is a program that will allow you to find the protein sequence for the DNA sequence (gene) you submit. Next click on blastx (translated nucleotide protein). Paste the DNA sequence into the box under “Entry Query Sequence.” Scroll down and click BLAST. The search may take a few seconds; the page will keep updating until the search is completed. You do not need to enter any…

Recommended textbooks for you

Human Anatomy & Physiology (11th Edition)

Biology

ISBN:

9780134580999

Author:

Elaine N. Marieb, Katja N. Hoehn

Publisher:

PEARSON

Biology 2e

Biology

ISBN:

9781947172517

Author:

Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:

OpenStax

Anatomy & Physiology

Biology

ISBN:

9781259398629

Author:

McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter

Publisher:

Mcgraw Hill Education,

Human Anatomy & Physiology (11th Edition)

Biology

ISBN:

9780134580999

Author:

Elaine N. Marieb, Katja N. Hoehn

Publisher:

PEARSON

Biology 2e

Biology

ISBN:

9781947172517

Author:

Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:

OpenStax

Anatomy & Physiology

Biology

ISBN:

9781259398629

Author:

McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter

Publisher:

Mcgraw Hill Education,

Molecular Biology of the Cell (Sixth Edition)

Biology

ISBN:

9780815344322

Author:

Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter

Publisher:

W. W. Norton & Company

Laboratory Manual For Human Anatomy & Physiology

Biology

ISBN:

9781260159363

Author:

Martin, Terry R., Prentice-craver, Cynthia

Publisher:

McGraw-Hill Publishing Co.

Inquiry Into Life (16th Edition)

Biology

ISBN:

9781260231700

Author:

Sylvia S. Mader, Michael Windelspecht

Publisher:

McGraw Hill Education

SEE MORE TEXTBOOKS