In order to identify cis-regulatory elements for the BRC2 gene in Arabidopsis thaliana, you develop genetically-engineered alleles in which different sections of the upstream region are deleted (dashed segments in the diagram below). You evaluate the amount of mRNA produced by each allele compared to the wild-type sequence. The results are shown in the diagram below in comparison with the wild-type sequence. Match the following cis-regulatory elements with the correct region:

In order to identify cis-regulatory elements for the BRC2 gene in Arabidopsis thaliana, you develop genetically-engineered alleles in which different sections of the upstream region are deleted (dashed segments in the diagram below). You evaluate the amount of mRNA produced by each allele compared to the wild-type sequence. The results are shown in the diagram below in comparison with the wild-type sequence. Match the following cis-regulatory elements with the correct region:

Name: 3.3 In order to identify cis-regulatory elements for the BRC2 gene in
Uploaded: 2024-03-20T06:42:55.000Z
Channel: Bartleby
Description: 3.3 In order to identify cis-regulatory elements for the BRC2 gene in Arabidopsis thaliana, you develop genetically-engineered allelesin which different sections of the upstream region are deleted (dashed segments in the diagram below). You evaluate

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter15: Genomes And Genomics

Section: Chapter Questions

Problem 13QP

See similar textbooks

Related questions

Q: What is triticale ?

A: Hybrid plants are produced when plant breeders intentionally cross-pollinate the two different…

Q: Some skeletal muscle fibers are “fast-twitch” fibers (white fibers), and others are “slow-twitch”…

A: Since you have asked multiple questions, we are eligible to answer only one question. Kindly repost…

Q: In what range would the blood pH of a heavy smoker with advanced emphysema fall: (a) less than 7.35;…

A: Emphysema is a disorder associated with the lungs which are characterized by damage to the alveoli,…

Q: 2.19 A conductive rubber cylindrical cord has a resistance/inch of about 350 2 per inch in its…

A: Conductive rubber is a type of rubber material that has been specially formulated to conduct…

Q: #21: Name the entire structure indicated by the bracket. #21

A: Plants have specialized tissues for the transportation of substances across different parts of the…

Q: Define M line

A: The myofibrils are long cylindrical fibers, arranged parallel, extending from one end of the fiber…

Q: Mannitol, a carbohydrate, is supplied as a 25% (w/v) solution. This hypertonic solution is given to…

A: Mannitol is a type of alcohol sugar formed from the monosaccharide carbohydrate, mannose. Mannitol…

Q: Toncity is the

Q: Large muscles, such as the muscles of the leg, have more muscle fibers than small muscles, such as…

A: The organ system that is involved majorly in the movement of the body is known as the muscular…

Q: State whether the following contraction, skeletal muscle is describing contraction, smooth muscle or…

A: The muscular system is one of the 11 major body organ systems of the body that aids in the…

Q: A motor unit from a skeletal muscle contains 1500 muscle fibers. Would this muscle be involved in…

A: Introduction: Gross motor skill is the ability that has been acquired during adulthood and forms the…

Q: OH NaOH

A: Fatty acids are long chain carboxylic acids. Here we are looking at the reaction between a free…

Q: Muscle soreness following depletion of oxygen during strenuous exercise is due in part to a buildup…

A: Introduction :- Muscle soreness is a side effect of the stress that exercise places on muscles. It's…

Q: Prozac, C17H18F3NO, is a widely used antidepressant that inhibits the uptake of serotonin by the…

A: Prozac Antidepressant sold as Fluoxetine inhibits the uptake of serotonin by the brain generally…

Concept explainers

Mitochondrial Mutations

A mitochondrion is a cellular organelle that serves as the site for cellular respiration and energy production (ATP or Adenosine Triphosphate). It is a double-membrane-bound organelle and is only present in eukaryotic cells.

Topic Video

Question

3.3

In order to identify cis-regulatory elements for the BRC2 gene in Arabidopsis thaliana, you develop genetically-engineered alleles
in which different sections of the upstream region are deleted (dashed segments in the diagram below). You evaluate the amount
of mRNA produced by each allele compared to the wild-type sequence. The results are shown in the diagram below in comparison
with the wild-type sequence.
Match the following cis-regulatory elements with the correct region:
a
b
present
d
deleted
% of wildtype
expression
100% (wt)
5%
150%
100%
<1%

Dragged and dropped options on the right-hand side will be automatically saved. For keyboard navigation... SHOW MORE ✓
No regulatory function
Silencer (binds repressor)
Enhancer (binds activator)
Core promoter
=
|||
|||
region A
region B
region D
region C

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions

Shown below is a schematic diagram illustrating a very short gene with 5000 bp region of an unknown Schizosaccharomyces pombe genome. (Note: Transcription starts at Transcription Start Site (TSS).) TSS 5. 3' 3 +1 (i) Name the specific regions that can be recognized by Transcription Factor IID (TF ID) and indicate the locations in the diagram above. (ii) List the mechanistic steps that can trigger the initiation of transcription by Transcription Factor IIH (TF IIH).
The chart below is a position specific scoring matrix (PSSM, a logarithmic transformed matrix) for a transcription factor binding site. (1). Evaluate a sequence “GACATTCA” to find out which segment of the sequence fits the binding site best. (2) What is the max score that a sequence can have with this PSSM? (3) What is the minimum score a sequence can have with this PSSM?
Given the following schematic for a gene and its associated regulatory regions, answer the following questions by placing the correct letter in the provided blanks please put in the correct letter for the questions What region would provide cell type-specific expression of genes? region What site would significantly increase gene expression rates? = region What region or regions of this gene’s coding sequence are expressed as amino acids = region
MRNA transcripts can be collected from cells, made to glow with a fluorescent dye, and added to a microarray chip containing spots with DNA sequences from each of these genes. (In the depiction of the microarrays below o indicates no binding of the MRNA, and • indicates binding.) HDL-R PFK CD3 After washing the chip, you then collect MRNAS from liver cells and add them to the same chip, which of the gene expression patterns would you expect to see? O A O B O D
In this chapter you were introduced to nonsense suppressor mutations in tRNA genes. However, suppressormutations also occur in protein-coding genes. Using thetertiary structure of the β subunit of hemoglobin shownin Figure 9-3(c), explain in structural terms how a mutation could cause the loss of globin protein function. Nowexplain how a mutation at a second site in the same protein could suppress this mutation and lead to a normalor near-normal protein.
The genes that encode the enzymes for arginine biosynthesis are located at several positions around the genome of E. coli, and they are regulated coordinately by a transcription regulator encoded by the ArgR gene. The activity of the Argr protein is modulated by arginine. upon binding arginine, Argr alters its conformation, dramatically changing its affinity for the DNA sequences in the promoters of the genes for the arginine biosynthetic enzymes. given that ArgR is a repressor protein, would you expect that ArgR would bind more tightly or less tightly to the DNA sequences when arginine is abundant? if ArgR functioned instead as an activator protein, would you expect the binding of arginine to increase or to decrease its affinity for its regulatory DNA sequences? explain your answers.
The codon change (Gly-12 to Val-12) in human rasH that convertsit to oncogenic rasH has been associated with many types ofcancers. For this reason, researchers would like to develop drugs toinhibit oncogenic rasH. Based on your understanding of the Rasprotein, what types of drugs might you develop? In other words,what would be the structure of the drugs, and how would theyinhibit Ras protein? How would you test the efficacy of the drugs?What might be some side effects?
Here is the model for TfR mRNA regulation (see Fig.), for your convenience. Orange protein (IRP or IREBP), when bound to RNA, protects mRNA from degradation. When Fe is bound to the protein, it cannot bind the mRNA. If you made a radioactive DNA probe specific to TfR mRNA and performed a Northern blot analysis (to detect mRNA) from cells treated with high iron (Fe) concentration what would you expect to see? (a) Low iron RNase A (b) High iron O High levels of RNase O Low levels of TfR mRNA Fe RNase O The result would be the same as untreated cells Degradation O High levels of IRP (or IREBP - iron responsive element binding protein) O High levels of TfR mRNA
The IMD2 promoter contains three upstream transcription start sites (TSS) that are utilized under high GTP conditions and a single downstream TSS (-106) that is normally only utilized under low GTP conditions. In a wild type cell, expression of IMD2 mRNA only occurs if transcription initiates from the -106 TSS. In 300 words or less, describe: 1.) The normal function of Ssl2, and 2.) why a mutation in Ssl2, that increases its catalytic rate, would allow expression of the IMD2 ORF under high GTP conditions. (Conditions under which the IMD2 ORF is NOT expressed in the wild type.)
Given the following schematic for a gene and its associated regulatory regions, answer the following questions by placing the correct letter in the provided blanks i need help find a letter for all the blanks
Briefly discuss (referring to the images provided) why mutant 2 fails to produce functional protein. Note that none of the mRNA transcribed from this gene is of the expected size; some of the mRNA molecules produced are 223 nucleotides shorter than expected, whilst others are 47 nucleotides longer than expected.
Searching the yeast Saccharomyces cerevisiae genome, researchers found approximately 4,000 DNA sites with a sequence which could potentially bind the yeast transcription factor GAL4. GAL4 activates the transcription of galactose genes. Yet there are only 10 GAL4-binding sites which control the genes necessary for galactose metabolism. The GAL4 binding sequence is CGGAT#AGAAGC*GCCG, where # is T, C or G, and * is C or T. In one chromatin immunoprecipitation experiment (ChIP), yeast growing on galactose were lysed, and subjected to cross-linking reagents which cross-linked transcription factors and activators to DNA. Next the DNA was sheared into small fragments, and antibodies to GAL4 were added. These antibodies coprecipitated the GAL4 and the DNA it was cross-linked to. The cross-linking was then chemically reversed, and the DNA was isolated, cloned into a library of plasmids and sequenced. Results showed that only 10 different DNA sequences had GAL4 bound. Since the…