A. Based on this data, draw a conclusion about the promoter elements that Caudal specifically upregulates. B. Dr. Juven-Gershon also tested Caudal activation of core promoter sequences from two other genes. Similar to the first experiment, one was DPE-dependent (from the E74B gene), and the other was TATA-dependent (from the Adh gene). However, these two core promoters lacked the BREU motif. The results from these additional core promoters are shown below. Based on these results and those shown above, propose a hypothesis that explains the differences in Caudal-mediated upregulation among all the different core promoters Dr. Juven-Gershon tested. C. To test your hypothesis from Question 3B, you plan perform the same method of gene reporter assays that Dr. Juven-Gershon has done. However, you will need to do some mutations to alter the core promoters. Make diagrams or clearly describe all the various core promoters mutants you wish to test. These include your controls, as well as any new mutations you would need to generate.
A. Based on this data, draw a conclusion about the promoter elements that Caudal specifically upregulates.
B. Dr. Juven-Gershon also tested Caudal activation of core promoter sequences from two
other genes. Similar to the first experiment, one was DPE-dependent (from the E74B
gene), and the other was TATA-dependent (from the Adh gene). However, these two core
promoters lacked the BREU motif. The results from these additional core promoters are
shown below. Based on these results and those shown above, propose a hypothesis that
explains the differences in Caudal-mediated upregulation among all the different core
promoters Dr. Juven-Gershon tested.
C. To test your hypothesis from Question 3B, you plan perform the same method of gene
reporter assays that Dr. Juven-Gershon has done. However, you will need to do some
mutations to alter the core promoters. Make diagrams or clearly describe all the various
core promoters mutants you wish to test. These include your controls, as well as any new
mutations you would need to generate.
D. Identify which constructs are important controls for your experiment, and briefly explain
each.
E. Describe and/or draw the results you expect to observe (i) if your hypothesis is correct
and (ii) if your explanation is incorrect.
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